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Preparation of Papain Complexes with Chitosan Microparticles and Evaluation of Their Stability Using the Enzyme Activity Level

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Pharmaceutical Chemistry Journal Aims and scope

Microparticles of medium- and high-molecular mass (MM) chitosan with and without ascorbic acid were obtained. Complexes of these microparticles with the plant enzyme papain were formed. The enzyme activity decreased by ?8% as compared to free papain upon formation of papain complexes with medium- and high-MM chitosan microparticles free of ascorbic acid. The papain activity increased by 18 and 10%, respectively, upon complexation in the presence of ascorbic acid. The free enzyme retained 15% of its catalytic activity after incubation for 168 h in Tris-HCl buffer (0.05 M, pH 7.5) at 37°C while the complexes with chitosan microparticles exhibited ~30% of their catalytic activity; the complex of papain with high-MM chitosan microparticles in the presence of ascorbic acid, 40%.

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Acknowledgments

The work was supported by a grant from the Russian Science Foundation (Project No. 21-74-20053).

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Authors and Affiliations

  1. Voronezh State University, 1 Universitetskaya Sq., Voronezh, 394018, Russia

    S. S. Ol’shannikova, Yu. A. Red’ko, M. S. Lavlinskaya, A. V. Sorokin, M. G. Holyavka & V. G. Artyukhov

  2. Voronezh State University of Engineering Technologies, 19 Revolyutsii Prosp., Voronezh, 394036, Russia

    M. S. Lavlinskaya & A. V. Sorokin

  3. Sevastopol State University, 33 Universitetskaya St., Sevastopol, 299053, Russia

    M. G. Holyavka

Authors
  1. S. S. Ol’shannikova
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  2. Yu. A. Red’ko
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  3. M. S. Lavlinskaya
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  4. A. V. Sorokin
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  5. M. G. Holyavka
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  6. V. G. Artyukhov
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Corresponding author

Correspondence to M. G. Holyavka.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 55, No. 11, pp. 51 – 55, November, 2021.

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Ol’shannikova, S.S., Red’ko, Y.A., Lavlinskaya, M.S. et al. Preparation of Papain Complexes with Chitosan Microparticles and Evaluation of Their Stability Using the Enzyme Activity Level. Pharm Chem J 55, 1240–1244 (2022). https://doi.org/10.1007/s11094-022-02564-8

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  • DOI: https://doi.org/10.1007/s11094-022-02564-8

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