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UHPLC, ATR-FTIR Profiling and Determination of 15 LOX, α-Glucosidase, Ages Inhibition and Antibacterial Properties of Citrus Peel Extracts

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A Correction to this article was published on 09 July 2022

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Diabetes mellitus (DM) has a worldwide prevalence with very alarming situation in low-income and developing countries including Pakistan. During this project, we investigated peels of two commonly used Citrus species, i.e., C. sinensis and hybrid Citrus (C. nobilis and C. delicense) for their antioxidant, antidiabetic, antiglycation, antimicrobial and anti-inflammatory activities. Initially the peels were dried and extracted with various solvents. Then, HPLC and ATR-FTIR analysis was performed and finally the extracts and fractions were analyzed for their biological properties. The HPLC and ATR-FTIR data showed the presence of phenolics and flavonoids. In C. sinensis peels, high concentration of phenolics (87.3 ± 0.11 mg/g GAE) were observed in ethyl acetate part followed by methanolic fraction (72.5 ± 0.12 mg/gGAE). Both extracts exhibited strong antioxidant activity, antimicrobial and anti-biofilm properties. In Advanced Glycation End Products (AGEs) assay, total extract of C. sinensis showed moderate (IC50 = 1.23 mg/mL) activity. All tested fractions were active in respect of α-glucosidase inhibition, whereas in 15-lox assay, only n-hexane fraction presented excellent activity (90%). On the other hand, in case of hybrid Citrus, a moderate inhibition was found in the case of total extract (IC50 = 0.25 mg/mL). Among fractions, highest inhibition was noticed for the methanolic part of crude extract (IC50 = 0.10 mg/mL). Both the total crude extract (IC50 = 0.23 mg/ml) and methanolic part of plant extract (IC50 =0.36 mg/ml) showed α-glucosidase inhibition, whereas only moderate 15-lox inhibition was observed.

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References

  1. WHO: Global Report on Diabetes, World Health Organization, Geneva (2016). Accessed on May 20, 2017.

  2. IDF Diabetes Atlas, (7th Edn.), International Diabetes Federation (IDF), Brussels, Belgium (2015).

  3. National Diabetes Fact Sheet: National Estimates and General Information on Diabetes and Prediabetes in the United States, Centers for Disease Control and Prevention (CDCP), Atlanta (2011).

  4. Diagnosis and Classification of Diabetes Mellitus, American Diabetes Association) ADA (2010), Diabetes Care, 33(1S) S62 – S69 (2010).

  5. A. Negre-Salvayre, N. Auge, V. Ayala, et al., Free Rad. Res., 44, 1125 – 1171(2010).

  6. R. M. Pollack, Y. Marc, R. Donath De Roith, and G. Leibowitz, Diabetes Care, 39 (Suppl. 2), S244 – S252 (2016).

  7. R. Zhou, A. Tardivel, B. Thorens, et al., Nat. Immunol. 11(3), 136 – 140 (2010).

    Article  CAS  Google Scholar 

  8. M. Böni-Schnetzler, S. Boller, S. Debray. et al. Endocrinology, 150(5), 5218 – 5229 (2009).

  9. L. Masters, A. Dunne, S. L. Subramanian, et al. Nat. Immunol., 11, 897 – 904 (2010).

    Article  CAS  Google Scholar 

  10. M. Y. Donath and S. E. Shoelson, Nat. Rev. Immunol. 11(2), 99 – 107 (2011).

    Article  Google Scholar 

  11. R. W. Thomsen, H. H. Hundborg, H. H. Lervang, et al., Diabetes Care, 27(3), 1143 – 1147(2004).

    Article  Google Scholar 

  12. S. Kawahito, H. Kitahata, and S. Oshita, World. J. Gastroenterol. 15, 4137 – 4142 (2009).

    Article  CAS  Google Scholar 

  13. L. Boyanova and I. Mitov, Expert. Rev. Anti-Infect. Ther., 11, 411 – 420 (2013).

    Article  CAS  Google Scholar 

  14. J. T. Cheng. Rev. J. Clin. Pharmacol., 40(5), 445 – 50 (2000).

    Article  CAS  Google Scholar 

  15. S. López-Varela and M. A. González-Gross, Eur. J. Clin. Nutr. 56. S29 (2002).

  16. R. Guimaraes, L. Barros, and J. C. Barreira, Food Chem. Toxicol., 48, 99 – 106 (2009).

    Article  Google Scholar 

  17. D. Ramful, E. Tarnus, P. Rondeau, et al., J. Agric. Food Chem., 58(20), 11119 – 11129 (2010).

    Article  CAS  Google Scholar 

  18. F. G. Gmitter and H. Xulan, Econ. Bot., 44(2), 267 – 277 (1990).

    Article  Google Scholar 

  19. M. A. Anagnostopoulou, P. Kefalas, E. Kokkalou, et al., Biomed. Chromatogr., 19(4), 138 – 148 (2005).

    Article  CAS  Google Scholar 

  20. C. Hu, J. Zawistowski, W. Ling, and D. D. Kitts. J. Agric. Food Chem., 51(18), 5271 – 5277 (2003).

    Article  CAS  Google Scholar 

  21. D. D. Kits and C. Hu, Annal. New York Acad. Sci., 1043(1), 501 – 512 (2005).

    Article  Google Scholar 

  22. M. H. Pan, C. S. Lai, and C. T. Ho, Food Funct., 1(1), 15 – 31 (2010).

    Article  CAS  Google Scholar 

  23. M. N. Abdullahi, N. Ilyas, and H. Ibrahim, Med. Plant. Res., 3, 37 – 43 (2013)

    Google Scholar 

  24. J. Chlopicka, P. Pasko, S. Gorinstein, et al., LWT-Food. Sci. Technol., 46, 548 – 555 (2012).

    Article  CAS  Google Scholar 

  25. M. S. Stankovic, Kragujevac J. Sci., 33, 63 – 72 (2011).

    Google Scholar 

  26. S. I Abdelwahab, A. B Abdul, M. M. Elhassan, et al., Int. J. Trop. Med, 4, 32 – 36 (2009).

  27. R. Y. Gan, X. R. Xu, F. L. Song, et al., J. Med. Plant. Res. 4, 2438 – 2444 (2010).

    Google Scholar 

  28. A. H. Weseler, K. Geiss, R. Saller and J. Retching, Pharmazie, 60, 498 – 502 (2005).

    CAS  PubMed  Google Scholar 

  29. T. Oja, P. San Martin Galindo, T. Taguchi, et al., Antimicrob. Agents Chemother., 59, 6046 – 6052 (2015).

  30. M. I. Choudhary, S. A. Shah, A. U. Rahman, et al., Steroids, 75(12), 956 – 966 (2000).

    Article  Google Scholar 

  31. S. N. Matsuura, T. Aradate, C. Sasaki, et al., J. Health Sci., 48, 520 – 526 (2002).

    Article  CAS  Google Scholar 

  32. K. E Malterud and K. M. Rydland. J. Agric. Food Chem., 48, 5576 – 5580 (2000).

  33. A. Catherine, R. E. Nicholas, and N. J. Miller, Free. Rad. Biol. Med., 20(7), 933 – 956 (1996).

    Article  Google Scholar 

  34. G. V. Gill, S. Redmond, F. Garratt, and R. Paisey. Diabetes Med., 11(2), 210 – 13 (2010).

    Article  Google Scholar 

  35. X. Zhang, X. Zhou, X. Liu, et al., J. Chromatogr. Sci.. 54(4), 574 – 582 (2016).

    Article  CAS  Google Scholar 

  36. Y. Sun, J. Wang, S. Gu, et al., Molecules. 15, 5378 – 5388 (2010).

    Article  CAS  Google Scholar 

  37. N. Yoshimizu, Y. Otani, Y. Saikawa, et al., Aliment. Pharm. Therap., 20, 95 – 101 (2004).

    Article  CAS  Google Scholar 

  38. N. Yee, L. G. Benning, V. R. Phoenix, ad F. G. Ferris. Environ. Sci. Technol., 38, 775 – 782 (2004).

  39. S. Y. Song, C. H. Kim, S. J. Im, and I. J. Kim. Food. Sci. Biotechnol.. 27(2), 367 – 374 (2018).

    CAS  PubMed  Google Scholar 

  40. V. Todorovic, M. Milenkovic, B. Vidovic, et al., J. Food. Sci., 82(4), 1020 – 1027 (2017).

    Article  CAS  Google Scholar 

  41. M. M. Cowan. Clin. Microbiol. Rev., 12(4), 564 – 582 (1999).

    Article  CAS  Google Scholar 

  42. A. Sati, S. Chandra, C. Sati, and O. P. Sati, Nat. Prod. Res., 31(6), 713 – 717 (2017).

    Article  CAS  Google Scholar 

  43. A. Upadhyay, E. Tuenter, A. Amin, et al., J. Funct. Foods, 11(2), 243 – 24 9(2014).

Download references

Acknowledgments

The Higher Education Commission of Pakistan (HEC) is acknowledged for grant (SRGP 1402) to Dr. Adnan Amin.

Conflict of Interest

The authors declare no conflict of Interest.

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Authors and Affiliations

  1. NPRL, Department of Pharmacognosy, Faculty of Pharmacy, Gomal University, D. I. Khan, 29050, Pakistan

    Muhammad Tayyab, Abdul Rafey,  Amanullah, Shahid Rasool & Fakhar ul Mahmood

  2. GCBB, Gomal University, D. I. Khan, 29050, Pakistan

    Muhammad Hanif

  3. Faculty of Agriculture, Gomal University, D. I. Khan, 29050, Pakistan

    Muhammad Mohibullah

  4. Gomal Medical College, D. I. Khan, 29050, Pakistan

    Nauman Reheem Khan & Nighat Aziz

  5. College of Pharmacy, University of Sargodha, Sargodha, Pakistan

    Adnan Amin

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  1. Muhammad Tayyab
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  4. Amanullah
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Correspondence to Adnan Amin.

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Tayyab, M., Hanif, M., Rafey, A. et al. UHPLC, ATR-FTIR Profiling and Determination of 15 LOX, α-Glucosidase, Ages Inhibition and Antibacterial Properties of Citrus Peel Extracts. Pharm Chem J 55, 176–186 (2021). https://doi.org/10.1007/s11094-021-02385-1

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