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LC–MS/MS characterization, antidiabetic, antioxidative, and antibacterial effects of different solvent extracts of Anamur banana (Musa Cavendishii)

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Abstract

The main objective of this study was to examine the phenolic compounds and the antibacterial, antioxidant, anti-α-glucosidase and anti-α-amylase activities of the different extracts (methanol, ethanol and hexane) of Musa cavendishii collected from the Anamur district in Turkey. LC–MS/MS was used to identify phenolic compounds. Quinic acid, acotinic acid, hesperidin and amentoflavone were identified in methanol extract. These phenolic compounds, excluding hesperidin, were also identified in the ethanol extract. Methanolic extract appeared the most active in all enzyme inhibition, antibacterial and antioxidative activity assays which is mainly due to its rich phenolic content. The methanol extract of banana showed the highest anti-α-glucosidase and anti-α-amylase activities with IC50 values of 5.45 ± 0.39 mg/mL, 9.70 ± 0.29 mg/mL, respectively. This study showed that methanol and ethanol extract, especially the methanol extract, have potential for use in the development of functional foods for reducing the diabetes and bacterial risks.

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References

  • Aurore G, Parfait B, Fahrasmane L. Bananas, raw materials for making processed food products. Trends in Food Science & Technology. 20: 78-91 (2009)

    Article  CAS  Google Scholar 

  • Ayoola IO, Gueye B, Sonibare MA, Abberton MT. Antioxidant activity and acetylcholinesterase inhibition of field and in vitro grown Musa L. species. Journal of Food Measurement and Characterization. 11: 488-499 (2017)

  • Begum YA, Deka SC. Chemical profiling and functional properties of dietary fibre rich inner and outer bracts of culinary banana flower. Journal of Food Science and Technology. 56(12): 5298-5308 (2019)

    Article  CAS  Google Scholar 

  • Boeing JS, Barizao ÉO, Silva BC, Montanher PF, de Cinque AV, Visentainer JV. Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chemistry Central Journal. 8(48): 1-9 (2014)

    Google Scholar 

  • Bouhafsoun A, Yilmaz MA, Boukeloua A, Temel H, Kaidharche M Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC–ESI-MS/MS. Food Science and Technology (Campinas). 38: 242-247 (2018)

  • Ertas A, Yilmaz MA, Firat M. Chemical profile by LC–MS/MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Natural Product Research. 29: 1-6 (2014)

    Google Scholar 

  • Fadhilah F, Jalani M, Mohamad S, Nazatul W, Shahidan S. Antibacterial effects of banana pulp extracts based on different extraction methods against selected microorganisms. Asian Journal of Biomedical and Pharmaceutical Sciences. 4(36): 14-19 (2014)

    Google Scholar 

  • Faostat. Statistical database of the food and agriculture organization of the united nations. http://faostat.fao.org/ (2017)

  • Fernandez-Agullo A, Pereira E, Freire MS, Valentao P, Andrade PB, Gonzalez Álvarez J, Pereira JA. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial Crops and Products. 42: 126-132 (2013)

    Article  CAS  Google Scholar 

  • Fowler F, Voyer B, Marino M, Finzel J, Veltri M, Wachter NM. Rapid screening and quantification of synthetic cannabinoids in herbal products with NMR spectroscopic methods. Analytical Methods. 7(18): 7907-7916 (2015)

    Article  CAS  Google Scholar 

  • Gulçin I. Antioxidant activity of food constituents: An overview. Archives of Toxicology. 86: 345-391 (2012)

    Article  Google Scholar 

  • Hoffmann JF, Carvalho IR, Barbieri RL, Rombaldi CV, Chaves FC Butia spp. (Arecaceae) LC-MS-based metabolomics for species and geographical origin discrimination. Journal of Agricultural and Food Chemistry. 65: 523−532 (2017)

    Article  CAS  Google Scholar 

  • Ibrahim EA, Desoukey SY, Hadad GM, Salam RA, Ibrahim A, Ahmed SA, Radwan, MM, Wanas AS, ElSohly MA. Analysis of cupressuflavone and amentoflavone from Cupressus sempervirens L. and its tissue cultured callus using HPLC-dad method. Pharmacy & Pharmacology International Journal. 5: 174–180 (2017)

    Google Scholar 

  • Ilokiassanga SB, Lewislujan LM, Laraespinoza CL, Gilsalido AA, Fernandezangulo D, Rubiopino JL, David DH. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC Research Notes. 8(396): 1-14 (2015)

    Google Scholar 

  • Jaber H, Baydoun E, El-Zein O, Kreydiyyeh SI. Anti-hyperglycemic effect of the aqueous extract of banana infructescence stalks in streptozotocin-induced diabetic rats. Plant Foods for Human Nutrition. 68: 83-89 (2013)

    Article  Google Scholar 

  • Kalita H, Boruah DC, Deori M, Hazarika A, Sarma R, Kumari S, Kandimalla R, Kotoky J, Devi R. Antidiabetic and antilipidemic effect of musa balbisiana root extract: a potent agent for glucose homeostasis in streptozotocin-ınduced diabetic rat. Frontiers in Pharmacology. 7(102): 1-11 (2016)

    Google Scholar 

  • Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Li GQ. A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase. Phytotherapy Research. 27: 1614-1620 (2013)

    Article  Google Scholar 

  • Khawas P, Deka SC. Encapsulation of natural antioxidant compounds from culınary banana by cocrystallization. Journal of Food Processing and Preservation. 41: 1-13 (2017)

    CAS  Google Scholar 

  • Kotha P, Badrib KR, Nagalapurama R, Allagaddaa R, Chippadaa AR. Anti-diabetic potential of the leaves of Anisomeles malabarica in streptozotocin induced diabetic rats. Cellular Physiology and Biochemistry. 43: 1689-1702 (2017)

    Article  CAS  Google Scholar 

  • Lehmann U, Jacobasch G, Schmiedl D. Characterization of resistant starch type III from banana (Musa acuminata). Journal of Agricultural and Food Chemistry. 50: 5236-5240 (2002)

    Article  CAS  Google Scholar 

  • Li W, Wang X, Zhang J, Zhao X, Wu Y, Tan S, Gao X. Multivariate analysis illuminates the effects of vacuum drying on the extractable and nonextractable polyphenols profile of loquat fruit. Journal of Food Science. 84: 726-737 (2019)

    Article  CAS  Google Scholar 

  • Linde K. St. John’s wort–an overview. Complementary Medicine Research. 16(3): 146-155 (2009)

    Google Scholar 

  • Liu S, Li D, Huang B, Chen Y, Lu X, Wang Y. Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves. Journal of Ethnopharmacology. 149(1): 263-269 (2013)

    Article  CAS  Google Scholar 

  • Nguyena VT, Bowyer MC, Vuonga QV, Altenaa IA, Scarlett CS. Phytochemicals and antioxidant capacity of Xao tam phan (Paramignya trimera) root as affected by various solvents and extraction methods. Industrial Crops and Products. 67: 192-200 (2015)

    Article  Google Scholar 

  • Ough CS, Amerine MA. Methods for analysis of musts and wines. New York: Wiley. 377 (1988)

    Google Scholar 

  • Ozcelik B, Kartal M, Orhan I. Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharmaceutical Biology. 49: 396-402 (2011)

    Article  CAS  Google Scholar 

  • Rezende CO, Oliveira LA, Oliveira BA, Almeida CG, Ferreira BS, Hyaric M, Carvalho GSL, Lourenço MCS, Batista M, Marchini FK, Silva VL, Diniz CG, Almeida MV. Synthesis and antibacterial activity of alkylated diamines and amphiphilic amides of quinic acid derivatives. Chemical Biology & Drug Design. 86: 344-350 (2015)

    Article  CAS  Google Scholar 

  • Sandjo LP, Marcus VP, dos Santos N, Moraes MH, Rodrigues LM, Dalmarco EM, Biavatti MW, Steindel M. NOx-, IL-1-, TNF-α-, and IL-6-inhibiting effects and trypanocidal activity of banana (Musa acuminata) bracts and flowers: UPLC-HRESI-MS detection of phenylpropanoid sucrose esters. Molecules 24: 4564 (2019)

    Article  CAS  Google Scholar 

  • Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects - A review. Journal of Functional Foods. 18: 820-897 (2015)

    Article  CAS  Google Scholar 

  • Shian T, Abdullah A, Musa KH, Ghani MM. Antioxidant properties of three banana cultivars (Musa acuminata ‘Berangan’, ‘Mas’ and ‘Raja’) extracts. Sains Malaysiana 41(3): 319-324 (2012)

    CAS  Google Scholar 

  • Sidhu JS, Zafar TA. Bioactive compounds in banana fruits and their health benefits. Food Safe Quality Food. 2: 183-188 (2018)

    Article  CAS  Google Scholar 

  • Silva AR, Cerdeira CD, Brito AR, Salles BCC, Ravazi GF, Moraes GDI, Rufino LR A, de Oliveira RBS, Santos GB. Green banana pasta diet prevents oxidative damage in liver and kidney and improves biochemical parameters in type 1 diabetic rats. Archives of Endocrinology Metabolism. 60: 355-366 (2016)

    Article  Google Scholar 

  • Singh B, Singh JP, Kaur A, Singh N. Bioactive compounds in banana and their associated health benefits—a review. Food Chemistry. 206: 1-11 (2016)

    Article  CAS  Google Scholar 

  • Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 19: 669-675 (2006)

    Article  CAS  Google Scholar 

  • Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutrition and Metabolis. 12(60): 1-20 (2015)

    Google Scholar 

  • Wulan DR, Utomo EP, Mahdi C. Antidiabetic activity of Ruellia tuberosa L., role of a-amylase inhibitor: in silico, in vitro, and in vivo approaches. Biochemistry Research International. 6: 1-9 (2015)

    Article  Google Scholar 

  • Yates CR, Zeng K, Miller DD, Thompson KE. Anti-inflammatory quinic acid derivatives for oral administration. Patent Application Publication. 17: 1-12 (2009)

    Google Scholar 

  • Yilmaz MA. Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: A detailed, robust and comprehensive LC–MS/MS method validation. Industrial Crops and Products. 149: 112347 (2020)

    Article  CAS  Google Scholar 

  • Zeng K, Thompson KE, Yates CR, Miller DD. Synthesis and biological evaluation of quinic acid derivatives as anti-inflammatory agents. Bioorganic & Medicinal Chemistry Letters. 19: 5458-5460 (2009)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the Commission for the Scientific Research Projects of University of Adana Alparslan Turkes Science and Technology, Turkey.

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Authors and Affiliations

  1. Department of Food Engineering, Faculty of Engineering, University of Adana Alparslan Turkes Science and Technology, 01250, Saricam, Adana, Turkey

    Aysun Şener Gedük & Fatma Zengin

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  1. Aysun Şener Gedük
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  2. Fatma Zengin
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Aysun Sener Geduk: Project administration, Investigation, Methodology, Formal analysis, Resources, Writing—review & editing. Fatma Zengin: Investigation, formal analysis, methodology, writing.

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Correspondence to Aysun Şener Gedük.

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Gedük, A.Ş., Zengin, F. LC–MS/MS characterization, antidiabetic, antioxidative, and antibacterial effects of different solvent extracts of Anamur banana (Musa Cavendishii). Food Sci Biotechnol 30, 1183–1193 (2021). https://doi.org/10.1007/s10068-021-00953-5

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