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Stability Indicating RP-HPLC Method for Simultaneous Determination of Pholcodine and Guaiacol in Pharmaceutical Syrup

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Pharmaceutical Chemistry Journal Aims and scope

Guaiacol (GUA) and pholcodine (PHO) were simultaneously determined in a pharmaceutical syrup using RP-HPLC with UV detection. The separation was performed using X-Bridge C8 column (100 mm × 4.6 mm, 2.5 μm particle size) and a mobile phase of acetonitrile – phosphate buffer (pH 6.8; 50 mM) (25:75, v/v) at a flow rate of 0.55 mL/min. The column temperature was adjusted to 35°C, and the detector was set to measure the absorbance at 238 nm. The ICH guidelines were followed to validate the method. The linearity was studied over 6.0 – 70 μg/mL and 4 – 420 μg/mL ranges for GUA and PHO, respectively. Linear relationships were obtained over the specified concentration ranges with correlation coefficients of 0.9989 for GUA and 0.9999 for PHO. The limits of detection were also determined and found to be 2 μg/mL for GUA and 1.2 μg/mL for PHO. The proposed method was applied for the determination of both drugs in Coughpent syrup; the results comply with the label claim (0.1572g/120mL for PHO and 0.0237g/120mL for GUA).

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References

  1. N. Sarma, G. Giancaspro, and J. Venema, Drug Test. Anal., 8, 418 – 423 (2016).

    Article  CAS  Google Scholar 

  2. C. M. Judkins, P. Teale, and D. J. Hall, Drug Test. Anal., 2, 417 – 420 (2010).

    Article  CAS  Google Scholar 

  3. I. A. Revelsky, E. S. Chernetsova, B. P. Luzyanin, et al, Drug Test. Anal., 2, 452 – 454 (2010).

    Article  CAS  Google Scholar 

  4. A. L. N. van Nuijs, A. Gheorghe, P. G. Jorens, et al, Drug Test. Anal., 6, 861 – 867 (2014).

    Article  CAS  Google Scholar 

  5. M. Sundström, A. Pelander, K. Simojoki, and I. Ojanperä, Drug Test. Anal., 8, 39 – 45 (2016).

    Article  Google Scholar 

  6. A. Maas, B. Madea, and C. Hess, Drug Test Anal, 1 – 18 (2017).

  7. T. Launiainen, I. Nupponen, E. Halmesmäki, and I. Ojanperä, Drug Test. Anal., 5, 529 – 533 (2013).

    Article  CAS  Google Scholar 

  8. A. Petkovska, H. Babunovska, and M. Stefova, Maced. J. Chem. Chem. Eng., 30, 139 – 150 (2011).

    Article  CAS  Google Scholar 

  9. B. Shi and H. Min, Chinese Pharm. Aff., 4, 23 – 29 (2011).

    Google Scholar 

  10. X. Wang, L. Zhang, D. Byrne, L. et al, Org. Lett., 16, 4090 – 4093 (2014).

    Article  CAS  Google Scholar 

  11. A. A. Moustafa, M. A. Hegazy, D. Mohamed, and O. Ali, J. AOAC Int., (2017).

  12. J. Acevska, A. Dimitrovska, G. Stefkov, et al, J. AOAC Int., 95, 399 – 405 (2012).

    Article  CAS  Google Scholar 

  13. A. A. Moustafa, H. Salem, M. Hegazy, and O. A. Mahmoud, J. Planar Chromatogr. – Mod. TLC, 28, 307 – 315 (2015).

    Article  CAS  Google Scholar 

  14. S. A. Mir, A. A. Ahangar, and A. S. Bhat, Int. J. PharmTech Res., 5, 341 – 348 (2013).

    CAS  Google Scholar 

  15. K. S. Bahçeci and J. Acar, Eur. Food Res. Technol., 225, 873 – 878 (2007).

    Article  Google Scholar 

  16. M. Hachiya, N. Asanome, T. Goto, and T. Noda, Japan Agric. Res. Q. JARQ, 43, 193 – 198 (2009).

    CAS  Google Scholar 

  17. Y. Hayasaka, G. A. Baldock, K. H. Pardon, et al, J. Agric. Food Chem., 58, 2076 – 2081 (2010).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, 31111, Egypt

    Fotouh R. Mansour

  2. Pharmaceutical Service Center, Faculty of Pharmacy, Tanta University, Tanta, 31111, Egypt

    Fotouh R. Mansour

  3. Research and Development, Glopal Napi Pharmaceuticals, 6th October City, Giza, 12511, Egypt

    Mostafa A. Khairy

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  1. Fotouh R. Mansour
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  2. Mostafa A. Khairy
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Correspondence to Fotouh R. Mansour.

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Mansour, F.R., Khairy, M.A. Stability Indicating RP-HPLC Method for Simultaneous Determination of Pholcodine and Guaiacol in Pharmaceutical Syrup. Pharm Chem J 53, 775–779 (2019). https://doi.org/10.1007/s11094-019-02078-w

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  • DOI: https://doi.org/10.1007/s11094-019-02078-w

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