Skip to main content
SpringerLink
Log in

HPLC-MS/MS Method for Determining Dabigatran in Human Blood Serum

  • Published:
Pharmaceutical Chemistry Journal Aims and scope

An HPLC-MS/MS method for determining dabigatran in human blood serum was developed. Samples were prepared by precipitation of proteins using MeOH followed by centrifugation and dilution with deionized H2O. The developed method had a simple sample-preparation procedure, short analysis time, and wide analytical range (from 1 to 1,000 ng/mL). This method was suitable for routine bioanalytical studies, in particular, therapeutic drug monitoring.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Fig. 7.
Fig. 8.
Fig. 9.
Fig. 10.

Similar content being viewed by others

References

  1. WHO. Cardiovascular Diseases. Informational Bulletin No. 317, January, 2015; http: //www.who.int/mediacentre/factsheets/fs317/ru/[http: //www.who.int/mediacentre/factsheets/fs317/en/].

  2. S. Stewart, C. L. Hart, D. J. Hole, and J. J. V. McMurray, Heart, 86(5), 516 – 521 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. A. S. Go, E. M. Hylek, K. A. Phillips, et al., J. Am. Med. Assoc., 285(18), 2370 – 2375 (2001).

    Article  CAS  Google Scholar 

  4. Atrial Fibrillation Investigators, “Risk factors for stroke and efficacy of antithrombotic therapy in atrial fibrillation: analysis of pooled data from five randomized clinical trials,” Arch. Intern. Med., 154, 1449 – 1457 (1994).

  5. P. Kirchhof, S. Benussi, D. Kotecha, et al., Eur. Heart J., 37(38), 2893 – 2962 (2016).

    Article  PubMed  Google Scholar 

  6. L. Wallentin, S. Yusuf, M. D. Ezekowitz, et al., Lancet, 376(9745), 975 – 983 (2010).

    Article  CAS  PubMed  Google Scholar 

  7. Pradaxa Prescribing Information; http: //www.docs.boehringer-ingelheim. com/Prescribing%20Information/PIs/Pradaxa/Pradaxa.pdf (accessed Nov. 10, 2007).

  8. S. Blech, T. Ebner, E. Ludwig-Schwellinger, et al., Drug Metab. Dispos., 36(2), 386 – 399 (2008).

    Article  CAS  PubMed  Google Scholar 

  9. W. Ageno, A. S. Gallus, A. Wittkowsky, et al., Chest, 141(2), e44S-88S (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. M. V. Huisman, G. Y. Lip, H. C. Diener, et al., Thromb. Haemostasis, 107(5), 838 (2012).

    Article  CAS  Google Scholar 

  11. A. J. Camm, G. Y. Lip, R. De Caterina, et al., Eur. Heart J., 33(21), 2719 – 2747 (2012).

    Article  PubMed  Google Scholar 

  12. K. H. Liesenfeld, T. Lehr, C. Dansirikul, et al., J. Thromb. Haemostasis, 9(11), 2168 – 2175 (2011).

    Article  CAS  Google Scholar 

  13. G. Pare, N. Eriksson, T. Lehr, et al., Circulation, 127(13), 1404 – 1412 (2013).

    Article  CAS  PubMed  Google Scholar 

  14. T. Ebner, K. Wagner, and W. Wienen, Drug Metab. Dispos., 38(9), 1567 – 1575 (2010).

    Article  CAS  PubMed  Google Scholar 

  15. F. Scaglione, Clin. Pharmacokinet., 52(2), 69 – 82 (2013).

    Article  CAS  PubMed  Google Scholar 

  16. T. Hellwig and M. Gulseth, Ann. Pharmacother., 47(11), 1478 – 1487 (2013).

    Article  CAS  PubMed  Google Scholar 

  17. Z. Y. Hu, R. B. Parker, and V. L. Herring, Anal. Bioanal. Chem., 405(5), 1695 – 1704 (2013).

    Article  CAS  PubMed  Google Scholar 

  18. P. A. Reilly, C. A. Conrad, R. A. Faaij, et al., Eur. Heart. J., 32(1), 6 (2011).

    Google Scholar 

  19. X. Delavenne, J. Moracchini, S. Laporte, et al., J. Pharm. Biomed. Anal., 58, 152 – 156 (2012).

    Article  CAS  PubMed  Google Scholar 

  20. M. Korostelev, K. Bihan, L. Ferreol, et al., J. Pharm. Biomed. Anal., 100, 230 – 235 (2014).

    Article  CAS  PubMed  Google Scholar 

  21. J. P. Antovic, M. Skeppholm, J. Eintrei, et al., Eur. J. Clin. Pharmacol., 69(11), 1875 – 1881 (2013).

    Article  CAS  PubMed  Google Scholar 

  22. J. Douxfils, L. Pochet, S. Lessire, et al., TrAC, Trends Anal. Chem., 84, 41 – 50 (2016).

    Article  CAS  Google Scholar 

  23. J. Douxfils, J. M. Dogne, F. Mullier, et al., Thromb. Haemostasis, 110(3), 543 – 549 (2013).

    CAS  Google Scholar 

  24. J. Douxfils, H. Mani, V. Minet, et al., BioMed Res. Int., 2015, Article ID 345138 (2015).

    Google Scholar 

  25. A. N. Mironov (ed.), Guideline for Drug Review, Vol. 1 [in Russian], Grifi K, Moscow (2013).

Download references

Author information

Authors and Affiliations

  1. Scientific Center for Expert Evaluation of Medical Products, Ministry of Health of the Russian Federation, 8/2 Petrovskii Blvd., Moscow, 127051, Russia

    T. A. Rodina, A. A. Aksenov, S. A. Belkov, A. V. Sokolov, A. B. Prokof’ev & G. V. Ramenskaya

  2. I. V. Davydovsky Municipal Clinical Hospital, Moscow City Department of Health, 11 Yauzskaya St., Moscow, 109240, Russia

    T. A. Rodina, E. S. Mel’nikov, S. A. Belkov & A. B. Prokof’ev

  3. I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), 8/2 Trubetskaya St., Moscow, 119991, Russia

    E. S. Mel’nikov, A. A. Aksenov, S. A. Belkov, A. V. Sokolov, A. B. Prokof’ev & G. V. Ramenskaya

Authors
  1. T. A. Rodina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. S. Mel’nikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Aksenov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. A. Belkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. V. Sokolov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. B. Prokof’ev
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. V. Ramenskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 51, No. 12, pp. 57 – 64, December, 2017.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rodina, T.A., Mel’nikov, E.S., Aksenov, A.A. et al. HPLC-MS/MS Method for Determining Dabigatran in Human Blood Serum. Pharm Chem J 51, 1129–1137 (2018). https://doi.org/10.1007/s11094-018-1753-1

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-018-1753-1

Keywords

Search

Navigation