An economic and rapid procedure for identification and quantitative determination of ascorbic acid using high-performance thin-layer chromatography (HPTLC) was developed. The optimum conditions for chromatography of vitamin C on a thin layer of absorbent with quantitative interpretation of the HPTLC data were established experimentally and justified theoretically. The proposed procedure was validated using medicinal plant raw materials such as nettle leaves and sea-buckthorn fruit. The procedure could be used for quality control of substances, single-component and complex preparations, plant materials, biologically active supplements, premixes, and products of the food and cosmetics industries.
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References
USSR State Pharmacopoeia, Xth Ed., Meditsina, Moscow (1968), pp. 483 – 485.
State Pharmacopoeia, XIth Ed., No. 1, Meditsina, Moscow (1987), pp. 217 – 221.
State Pharmacopoeia of the Russian Federation, XIIth Ed., Part 1, Nauchnyi Tsentr Ekspertizy Sredstv Meditsinskogo Naznacheniya, Moscow (2008).
N. I. Grinkevich and L. N. Safronich (eds.), Chemical Analysis of Medicinal Plants [in Russian], Vysshaya Shkola, Moscow (1983).
Ya. M. Perel’man, Analysis of Dosage Forms [in Russian], Gos. Izd. Med. Lit., Leningrad (1961), pp. 389 – 391.
Yu. M. Ostrovskii (ed.), Experimental Vitaminology [in Russian], Nauka i Tekhnika, Minsk (1979), pp. 80 – 129.
A. I. Luttseva and L. G. Maslov, Khim.-farm. Zh., 33(9), 30 – 35 (1999); Pharm. Chem. J., 33(9), 490 – 498 (1999).
S. G. Abdullina, N. M. Agapova, R. Sh. Khaziev, and S. A. Sidullina, Farmatsiya, No. 2, 17 – 19 (2011).
E. V. Sergunova, A. I. Marakhova, and A. S. Avrach, Farmatsiya, No. 4, 8 – 11 (2013).
A. I. Marakhova, O. A. Supakova, N. N. Fedorovskii, et al., Farmatsiya, No. 1, 24 – 27 (2013).
A. A. Zemlyanukhin and L. A. Zemlyanukhin, Large Practicum on Plant Physiology and Biochemistry: Student Aide [in Russian], VGU, Voronezh (1996).
I. M. Korenman, Photometric Analysis. Methods for Determining Organic Compounds [in Russian], Khimiya, Moscow (1970).
V. S. Kartashov, Vopr. Biol. Med. Farm. Khim., No. 3, 44 – 46 (1998).
GOST 24556-89. Products of fruit and vegetable processing. Methods for determining vitamin C.
D. A. Borisova and D. M. Popov, Farmatsiya, No. 3, 22 – 24 (2013).
T. S. Popova and O. G. Potanina, Farmatsiya, No. 2, 11 – 13 (2013).
GOST 31643-2012. Juice products. Determination of ascorbic acid by high-performance liquid chromatography.
J. G. Kirchner, Techniques of Chemistry, Vol. 14: Thin-Layer Chromatography, 2nd Ed., Wiley-Interscience, New York (1978), 1137 pp [Russian translation, Mir, Moscow (1981), pp. 402 – 407].
M. Sarsunova, C. Michalec, and C. Schwarz, Thin-Layer Chromatography in Pharmacy and Clinical Biochemistry [in Slovak], Obzor, Bratislava (1968) [Russian translation, Mir, Moscow (1980), p. 610].
Yu. V. Tanskaya and O. I. Popova, Proceedings of the Scientific-Practical Conference “Pharmacy from Century to Century” [in Russian], Part III, St. Petersburg (2008), pp. 151 – 155.
V. V. Vershinina and V. A. Kurkin, Med. Al’manakh, No. 2, 144 – 146 (2011).
G. S. Chakraborthy, J. Young Pharm., 1(1), 82 – 85 (2009); doi: https://doi.org/10.4103/0975–1483.51878.
F. Buhl, B. Szpikowska-Sroka, and M. Galkowska, J. Planar Chromatogr., 18; DOI: https://doi.org/10.1556/JPC.18.2005.5.6.
M. Abdel-Kader, J. Planar Chromatogr., 26(4), 336 (2013); DOI: https://doi.org/10.1556/JPC.26.2013.4.8.
E. L. Ponder, B. Fried, and J. Sherma, Acta Chromatogr., 14, 70 – 81 (2004).
R. R. R. Kannan, R. Arumugam, S. Meenakshi, and P. Anantharaman, Int. J. ChemTech Res., 2(3), 1526 – 1530 (2010).
V. Hima, S. R. Kumar, N. Duganath, and N. Devanna, PharmaChem, 5(3), 8 – 17 (2013).
K. M. Younes, M. A. Basha, Y. Maissa, et al., PharmaChem, 6(2), 111 – 121 (2014).
M. S. Kondawar, K. G. Kamble, and D. S. Mali, Int. J. PharmTech Res., 3(3), 1593 – 1599 (2011).
S. Himesh, A. K. Singhai, S. Sarvesh, et al., Int. J. Curr. Pharm. Res., 4(1), 43 – 47 (2012).
Z. Zloch and E. Ginter, Z. Klin. Chem. Klin. Biochem., 3, 302 – 305 (1970).
S. Gyan, L. Narain, and R. Road, Int. J. Pharm. Pharm. Sci., 4(3), 144 – 147 (2012).
A. Mohammad, S. Sharma, and S. A. Bhawani, Indian J. Chem. Technol., 16, 344 – 350 (2009).
State Pharmacopoeia of the Russian Federation, XIIIth Ed., in 3 vol., Ministerstvo Zdravookhraneniya Rossiiskoi Federatsii, Moscow (2015); http://www.femb.ru/feml.
O. B. Rudakov, I. A. Vostrov, S. V. Fedorov, et al., The Chromatographer’s Companion. Liquid Chromatography Methods [in Russian], Vodolei, Voronezh (2004).
F. Geiss, Fundamentals of Thin Layer Chromatography, A. Huthig, Heidelberg, New York (1987).
M. V. Gavrilin and S. P. Senchenko, Validation of Analytical Procedures [in Russian], PGFA, Pyatigorsk (2008).
E. A. Vasilenko, T. D. Mezenova, O. I. Popova, and A. B. Dmitriev, Farmatsiya, No. 1, 16 – 19 (2013).
O. V. Trineeva, E. F. Safonova, I. I. Safonova, A. I. Slivkin, et al., RU Pat. 2,581,456, IPC 601N30 / 94, “Method for identification and quantitative determination of ascorbic acid” [in Russian], applicant and patent holder: State Educational Institution of Higher Professional Education Voronezh State University; Appl. No. 2014141086 / 28; Oct. 10, 2014; Publ. Apr. 20, 2016; Byull. Izobret., No. 11 (2014).
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 51, No. 10, pp. 58 – 64, October, 2017.
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Trineeva, O.V., Safonova, E.F. & Slivkin, A.I. Method Development for Quantitative Determination of Ascorbic Acid by High-Performance Thin-Layer Chromatography. Pharm Chem J 51, 938–944 (2018). https://doi.org/10.1007/s11094-018-1719-3
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DOI: https://doi.org/10.1007/s11094-018-1719-3