Butoconazole nitrate pharmacokinetics studied by capillary electrophoresis

In view of the wide use of butoconazole nitrate in practical gynecology, a method for determining this drug in the blood of experimental animals has been developed based on capillary electrophoresis. Samples were prepared using sedimentation of plasma proteins by acetonitrile. The separation was carried out at 27°C in a quartz capillary (75 μm diameter, 65 cm working length) at 20 kV. The leading electrolyte was a phosphate buffer solution at pH 3.6. Detection was performed by spectrophotometry at 210 nm. The proposed technique was used to study the pharmacokinetics of butoconazole nitrate in rats upon a single intraperitoneal injection at a dose of 80 mg/kg. The kinetic curves of butoconazole nitrate concentration in the blood were constructed and were typical of drug removal with bile.