Abstract
Temporary biocompatible and degradable cell scaffolds - the new weapon of tissue engineering in the face of personalized medicine are emerging as one of the most powerful tools for guided self-regeneration of injured, diseased or malfunctioning tissues. In the current study, CPA Ti:sapphire fs laser system (τ = 150 fs, λ = 800 nm, ѵ=0.5 kHz) was used for surface modification of Poly Lactic Acid (PLA) temporary cell scaffolds at fluence F = 0.8 J/cm2 and scanning velocity V = 3.8 mm/s. Additional thin layer of chitosan (Ch)/hydroxyapatite (HAp) (up to 30 ÷ 60 nm thickness) was deposited on the laser-modified PLA matrices by spin coating method for cell scaffolds surface functionalization. In order to observe the complementary impact of fs structuring and spin coating on the PLA scaffolds’ properties, both surface modification methods were applied on the prepared by compression molding PLA samples. Each laser processed sample was analyzed in respect of the corresponding control – laser-treated and untreated PLA surface, spin-coated with Ch or HAp. The microstructured scaffolds were characterized by SEM, EDX, FTIR, roughness, and WCA analyses. The results obtained from characterization of scaffold properties, show that such combined methods application for functionalization of the bone PLA scaffolds could be applied to improve the biocompatibility of the as created PLA-chitosan and PLA- hydroxyapatite hybrid cell matrices.
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This research was funded by BULGARIAN NATIONAL SCIENCE FUND (NSF) under grant number No. KP-06-H48/6 (2020–2023), „Development of hybrid functional micro/nanoporous biomaterial scaffolds by ultra-fast laser modification”; EUROPEAN UNION’S H2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 861138 and H2020 FET Open METAFAST Grant Agreement No. 899673.
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Angelova, L., Daskalova, A., Filipov, E. et al. Analysis of femtosecond microstructured Poly Lactic Acid temporary cell scaffolds, spin-coated with Chitosan or Hydroxyapatite. Opt Quant Electron 54, 721 (2022). https://doi.org/10.1007/s11082-022-03970-8
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DOI: https://doi.org/10.1007/s11082-022-03970-8