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Gain of circBRAF Represses Glioma Progression by Regulating miR-1290/FBXW7 Axis

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Abstract

Dysregulated circular RNAs (circRNAs) have been confirmed to partake in the modulation of the glioma progression. Here, we intended to explore the role of circBRAF in glioma and the possible action mechanism. The expression levels of circBRAF, microRNA (miR)-1290 and F-box and WD repeat domain containing 7 (FBXW7) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or western blot. Cell viability was assessed by 3-(4, 5)-dimethylthiazole-2-y1)-2, 5-biphenyl tetrazolium bromide (MTT) assay. Cell cycle distribution was determined by flow cytometry. Cell migration and invasion were evaluated through Trans-well assay. Related protein levels were detected by western blot. Targeted relation among circBRAF, miR-1290 and FBXW7 was validated by dual-luciferase reporter, RNA immunoprecipitation (RIP) and pull-down assays. Xenograft model was constructed to explore the function of circBRAF in vivo. Expression of circBRAF and FBXW7 was decreased in glioma tissues and cells. Upregulation of circBRAF inhibited glioma cell proliferation and metastasis in vitro. MiR-1290 was upregulated in glioma, which was sponged by circBRAF. Besides, circBRAF elevated FBXW7 expression by targeting miR-1290. Introduction of miR-1290 or FBXW7 knockdown could counteract the inhibitory effects of circBRAF upregulation on the malignant phenotypes of glioma cells. Overexpression of circBRAF repressed the tumor growth in vivo. Upregulation of circBRAF suppressed glioma evolvement in vitro and in vivo by regulating miR-1290/FBXW7 axis, broadening the cognition of glioma progression.

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References

  1. McComb RD, Burger PC (1985) Pathologic analysis of primary brain tumors. Neurol Clin 3:711–728

    Article  CAS  Google Scholar 

  2. Lapointe S, Perry A, Butowski NA (2018) Primary brain tumours in adults. Lancet 392:432–446

    Article  Google Scholar 

  3. Ostrom QT, Gittleman H, Liao P, Rouse C, Chen Y, Dowling J, Wolinsky Y, Kruchko C, Barnholtz-Sloan J (2014) CBTRUS statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2007–2011. Neuro Oncol 16(Suppl 4):iv1–iv63

    Article  Google Scholar 

  4. Chen H, Chen G, Li G, Zhang S, Chen H, Chen Y, Duggan D, Hu Z, Chen J, Zhao Y, Zhao Y, Huang H, Zheng SL, Trent JM, Yu L, Jiang D, Mo Z, Wang H, Mou Y, Jiang T, Mao Y, Xu J, Lu D (2019) Two novel genetic variants in the STK38L and RAB27A genes are associated with glioma susceptibility. Int J Cancer 145:2372–2382

    Article  CAS  Google Scholar 

  5. Tanaka S, Louis DN, Curry WT, Batchelor TT, Dietrich J (2013) Diagnostic and therapeutic avenues for glioblastoma: no longer a dead end? Nat Rev Clin Oncol 10:14–26

    Article  CAS  Google Scholar 

  6. Little MP, de Vathaire F, Shamsaldin A, Oberlin O, Campbell S, Grimaud E, Chavaudra J, Haylock RG, Muirhead CR (1998) Risks of brain tumour following treatment for cancer in childhood: modification by genetic factors, radiotherapy and chemotherapy. Int J Cancer 78:269–275

    Article  CAS  Google Scholar 

  7. Mazurek M, Litak J, Kamieniak P, Kulesza B, Jonak K, Baj J, Grochowski C (2020) Metformin as potential therapy for high-grade glioma. Cancers (Basel) 12(1):210

    Article  CAS  Google Scholar 

  8. Meng S, Zhou H, Feng Z, Xu Z, Tang Y, Li P, Wu M (2017) CircRNA: functions and properties of a novel potential biomarker for cancer. Mol Cancer 16:94

    Article  Google Scholar 

  9. Hao Z, Hu S, Liu Z, Song W, Zhao Y, Li M (2019) Circular RNAs: functions and prospects in glioma. J Mol Neurosci 67:72–81

    Article  CAS  Google Scholar 

  10. Yang M, Li G, Fan L, Zhang G, Xu J, Zhang J (2019) Circular RNA circ_0034642 elevates BATF3 expression and promotes cell proliferation and invasion through miR-1205 in glioma. Biochem Biophys Res Commun 508:980–985

    Article  CAS  Google Scholar 

  11. Li G, Yang H, Han K, Zhu D, Lun P, Zhao Y (2018) A novel circular RNA, hsa_circ_0046701, promotes carcinogenesis by increasing the expression of miR-142-3p target ITGB8 in glioma. Biochem Biophys Res Commun 498:254–261

    Article  CAS  Google Scholar 

  12. Li F, Ma K, Sun M, Shi S (2018) Identification of the tumor-suppressive function of circular RNA ITCH in glioma cells through sponging miR-214 and promoting linear ITCH expression. Am J Transl Res 10:1373–1386

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Zhu J, Ye J, Zhang L, Xia L, Hu H, Jiang H, Wan Z, Sheng F, Ma Y, Li W, Qian J, Luo C (2017) Differential expression of circular RNAs in glioblastoma multiforme and its correlation with prognosis. Transl Oncol 10:271–279

    Article  Google Scholar 

  14. Lynam-Lennon N, Maher SG, Reynolds JV (2009) The roles of microRNA in cancer and apoptosis. Biol Rev Camb Philos Soc 84:55–71

    Article  Google Scholar 

  15. Mazurek M, Litak J, Kamieniak P, Osuchowska I, Maciejewski R, Roliński J, Grajkowska W, Grochowski C (2020) Micro RNA molecules as modulators of treatment resistance, immune checkpoints controllers and sensitive biomarkers in glioblastoma multiforme. Int J Mol Sci 21(4):1507

    Article  CAS  Google Scholar 

  16. Zhou Q, Liu J, Quan J, Liu W, Tan H, Li W (2018) MicroRNAs as potential biomarkers for the diagnosis of glioma: a systematic review and meta-analysis. Cancer Sci 109:2651–2659

    Article  CAS  Google Scholar 

  17. Dong Q, Yuan G, Liu M, Xie Q, Hu J, Wang M, Liu S, Ma X, Pan Y (2019) Downregulation of microRNA-374a predicts poor prognosis in human glioma. Exp Ther Med 17:2077–2084

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Wang LQ, Sun W, Wang Y, Li D, Hu AM (2019) Downregulation of plasma miR-124 expression is a predictive biomarker for prognosis of glioma. Eur Rev Med Pharmacol Sci 23:271–276

    PubMed  Google Scholar 

  19. Yan L, Cai K, Sun K, Gui J, Liang J (2018) MiR-1290 promotes proliferation, migration, and invasion of glioma cells by targeting LHX6. J Cell Physiol 233:6621–6629

    Article  CAS  Google Scholar 

  20. Yeh CH, Bellon M, Nicot C (2018) FBXW7: a critical tumor suppressor of human cancers. Mol Cancer 17:115

    Article  Google Scholar 

  21. Peng G, Yang C, Liu Y, Shen C (2019) miR-25-3p promotes glioma cell proliferation and migration by targeting FBXW7 and DKK3. Exp Ther Med 18:769–778

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Wang D, Yang S, Wang H, Wang J, Zhang Q, Zhou S, He Y, Zhang H, Deng F, Xu H, Zhong S, Fu L, Tang J (2018) The progress of circular RNAs in various tumors. Am J Transl Res 10:1571–1582

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Yuan Y, Jiaoming L, Xiang W, Yanhui L, Shu J, Maling G, Qing M (2018) Analyzing the interactions of mRNAs, miRNAs, lncRNAs and circRNAs to predict competing endogenous RNA networks in glioblastoma. J Neurooncol 137:493–502

    Article  Google Scholar 

  24. Thomson DW, Dinger ME (2016) Endogenous microRNA sponges: evidence and controversy. Nat Rev Genet 17:272–283

    Article  CAS  Google Scholar 

  25. Sun Y, Ju XL, Li D, Zhou PP, Li X, Luo RH (2019) miR-1290 promotes proliferation and suppresses apoptosis in acute myeloid leukemia by targeting FOXG1/SOCS3. J Biol Regul Homeost Agents 33:1703–1713

    CAS  PubMed  Google Scholar 

  26. Qin WJ, Wang WP, Wang XB, Zhang XT, Du JD (2019) MiR-1290 targets CCNG2 to promote the metastasis of oral squamous cell carcinoma. Eur Rev Med Pharmacol Sci 23:10332–10342

    PubMed  Google Scholar 

  27. Jin JJ, Liu YH, Si JM, Ni R, Wang J (2018) Overexpression of miR-1290 contributes to cell proliferation and invasion of non small cell lung cancer by targeting interferon regulatory factor 2. Int J Biochem Cell Biol 95:113–120

    Article  CAS  Google Scholar 

  28. Liu X, Ma J, Xu F, Li L (2018) TINCR suppresses proliferation and invasion through regulating miR-544a/FBXW7 axis in lung cancer. Biomed Pharmacother 99:9–17

    Article  CAS  Google Scholar 

  29. Xia W, Zhou J, Luo H, Liu Y, Peng C, Zheng W, Ma W (2017) MicroRNA-32 promotes cell proliferation, migration and suppresses apoptosis in breast cancer cells by targeting FBXW7. Cancer Cell Int 17:14

    Article  Google Scholar 

  30. Liu S, Liu H, Deng M, Wang H (2020) MiR-182 promotes glioma progression by targeting FBXW7. J Neurol Sci 411:116689

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Neurosurgery, Yingtan People’s Hospital, No. 116, Shenglixi Road, Yingtan, 335000, Jiangxi, China

    Jinchao Zhang, Zhi Chen, Xinjun Liu, Chonglin Yang & Donggen Xie

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  1. Jinchao Zhang
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  2. Zhi Chen
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  3. Xinjun Liu
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Correspondence to Jinchao Zhang.

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Zhang, J., Chen, Z., Liu, X. et al. Gain of circBRAF Represses Glioma Progression by Regulating miR-1290/FBXW7 Axis. Neurochem Res 46, 1203–1213 (2021). https://doi.org/10.1007/s11064-021-03259-4

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  • DOI: https://doi.org/10.1007/s11064-021-03259-4

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