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High-Dose Tanshinone IIA Suppresses Migration and Proliferation While Promoting Apoptosis of Astrocytoma Cells Via Notch-1 Pathway

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Abstract

Malignant astrocytoma is the most common malignant tumor with strong invasion in the central nervous system. Tanshinone IIA is an effective compound to suppress cell proliferation and promote cell apoptosis. However, there is little research about the role of tanshinone IIA in the treatment of astrocytoma. This study aimed to investigate the effect of tanshinone IIA on migration, proliferation and apoptosis of astrocytoma cells. The efficacy of tanshinone IIA on migration, proliferation and apoptosis of astrocytoma cells were evaluated by flow cytometry and the assays of plate clone formation, CCK-8, wound healing and transwell migration. The protein molecule and signaling pathway were detected by western blot. High-dose tanshinone IIA suppressed migration and proliferation of astrocytoma cells while promoting apoptosis of astrocytoma cells. The western blot results showed that there were high Notch-1 protein expression and low c-Myc, MMP-9 and Bcl-2 activation in the high-dose tanshinone IIA group compared with the control group. High-dose tanshinone IIA suppresses astrocytoma cell proliferation, migration while promoting apoptosis through Notch-1 pathway. Tanshinone IIA may be used to develop new drugs for the treatment of astrocytoma.

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Authors and Affiliations

  1. Neurology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China

    Wanliang Dong & Yanjie Jia

  2. Vasculocardiology Deparment, Zhengzhou People’s Hospital, Zhengzhou, Henan, China

    Yuankun Zhang

  3. Department of Anatomy of the School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China

    Xuemei Chen

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  1. Wanliang Dong
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  2. Yuankun Zhang
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  3. Xuemei Chen
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  4. Yanjie Jia
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Correspondence to Yanjie Jia.

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Dong, W., Zhang, Y., Chen, X. et al. High-Dose Tanshinone IIA Suppresses Migration and Proliferation While Promoting Apoptosis of Astrocytoma Cells Via Notch-1 Pathway. Neurochem Res 43, 1855–1861 (2018). https://doi.org/10.1007/s11064-018-2601-0

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  • DOI: https://doi.org/10.1007/s11064-018-2601-0

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