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The Effects of Granulocyte-Colony Stimulating Factor on Regeneration in Nerve Crush Injuries in Rats

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Abstract

Granulocyte-colony stimulating factor (G-CSF) is widely known to have a neuroprotective effect, but its effects on function and morphology in mechanical nerve injury are not well understood. The aim of this study was to confirm the time course of the functional changes and morphological effects of G-CSF in a rat model of nerve crush injury. Twelve-eight rats were divided into three group: sham-operated control group, G-CSF-treated group, and saline treated group. 2 weeks after the nerve crush injury, G-CSF was injected for 5 days. After 4 weeks, functional tests such as motor nerve conduction velocity (MNCV), mechanical and cold allodynia tests, and morphological studies were performed. G-CSF-treated rats had significantly improved nerve function including MNCV and mechanical and cold allodynia. In addition, G-CSF-treated rats had significantly higher the density of myelinated fibers than saline-treated rats. In conclusion, we found that 100 μg/kg administration of G-CSF promoted long-term functional recovery in a rat model of nerve crush injury.

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Acknowledgments

This work was supported by the research fund of Hanyang University HY-2014. The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea

    Yi-Sun Song, Jun-Ho Joe, Hyun-Woo Joo, In-Hwa Park, Ki-Jun Kim & Kyung-Soo Kim

  2. Cardiology Division, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea

    Guang-Yin Shen, Jeong Hun Shin & Kyung-Soo Kim

  3. Department of Cardiology, Sungae Hospital, Seoul, South Korea

    Yonggu Lee

  4. Department of Thoracic and Cardiovascular Surgery, Hanyang University College of Medicine, Seoul, South Korea

    Hyuck Kim

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  1. Yi-Sun Song
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  2. Jun-Ho Joe
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Correspondence to Kyung-Soo Kim.

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Song, YS., Joe, JH., Joo, HW. et al. The Effects of Granulocyte-Colony Stimulating Factor on Regeneration in Nerve Crush Injuries in Rats. Neurochem Res 41, 1645–1650 (2016). https://doi.org/10.1007/s11064-016-1879-z

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  • DOI: https://doi.org/10.1007/s11064-016-1879-z

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