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Time-Dependent Increase of Chitinase1 in APP/PS1 Double Transgenic Mice

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Abstract

It is reported that chitinase1 increases in Alzheimer’s disease (AD). However, the alteration of chitinase1 in the progress of AD is still unclear. Thus, we designed the present study to detect chitinase1 level in different stages of APP/PS1 double transgenic mice. Experimental models were APP/PS1 double transgenic mice with 4, 12 and 22 months. Cognitive function was detected by Morris water maze test in APP/PS1 mice as well as controls. ELISA and the quantitative RT-PCR were used to detect chitinase1 level in different groups. The study displayed that expression of chitinase1 gradually increased in a time-dependent manner in APP/PS1 mice, while there were no statistical differences among the wild-type mice in varies ages. Moreover, chitnase1 increased significantly in APP/PS1 mice aged 12 and 22 months compared with the age matched wild-type group, respectively. However, no difference of chitnase1 was found between 4 months-old APP/PS1 mice and wild-type mice. Comparing with the age matched wild type group, the consequences of mRNA on the increase in chitnase1 is in accordance with protein in APP/PS1 mice. Furthermore, Morris water maze showed that 4 months-old APP/PS1 mice have normal spatial learning and impaired spatial memory; both spatial learning and spatial memory in 12 and 22 months-old APP/PS1 mice were declined. Time-dependent increase of chitnase1 in APP/PS1 double transgenic mice indicates that the level of chitinase1 is associated with decline of cognition. Therefore, chitinase1 might be a biomarker of disease progression in AD.

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Acknowledgments

This study is supported by grants from National Key Clinical Specialties Construction Program of China (No. [2013]544), and Application Program of Chongqing Science & Technology Commission (cstc2014yykfA110002). None of the authors declared a conflict of interest.

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    Authors and Affiliations

    1. Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

      Qian Xiao, Rui Shi, Yan Zou, Yinshi Du, Man Zhang & Yang Lü

    2. Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China

      Wenxiu Yang & Weihua Yu

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    1. Qian Xiao
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    Correspondence to Yang Lü.

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    Qian Xiao and Rui Shi have contributed equally to this work.

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    Xiao, Q., Shi, R., Yang, W. et al. Time-Dependent Increase of Chitinase1 in APP/PS1 Double Transgenic Mice. Neurochem Res 41, 1604–1611 (2016). https://doi.org/10.1007/s11064-016-1874-4

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