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1,8-Cineole (Eucalyptol) Mitigates Inflammation in Amyloid Beta Toxicated PC12 Cells: Relevance to Alzheimer’s Disease

Neurochemical Research volume 39, pages 344–352 (2014)Cite this article

Abstract

Inflammatory process has a fundamental role in the pathogenesis of Alzheimer’s disease and insoluble amyloid beta deposits and neurofibrillary tangles provide the obvious stimuli for inflammation. The present study demonstrate the effect of pretreatment of 1,8-cineole (Cin) on inflammation induced by Aβ(25–35) in differentiated PC12 cells. The cells were treated with Cin at different doses for 24 h and then replaced by media containing Aβ(25–35) for another 24 h. The cell viability was decreased in Aβ(25–35) treated cells which was significantly restored by Cin pretreatment. Cin successfully reduced the mitochondrial membrane potential, ROS and NO levels in Aβ(25–35) treated cells. Cin also lowered the levels of proinflammatory cytokines TNF-α, IL-1β and IL-6 in Aβ(25–35) treated cells. Moreover, Cin also succeeded in lowering the expression of NOS-2, COX-2 and NF-κB. This study suggests the protective effects of Cin on inflammation and provides additional evidence for its potential beneficial use in therapy as an anti-inflammatory agent in neurodegenerative disease.

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Acknowledgments

The author (A.K.) thanks Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi for the financial assistance. We highly appreciate Dharamvir Singh and Mohd Idris for technical assistance. We also acknowledge SABiosciences as the source of the original pathway map.

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Author notes

  1. Andleeb Khan

    Present address: Biomedical Research Unit, Medical Research Center, Jazan University, Jazan, 45142, Kingdom of Saudi Arabia

  2. Hayate Javed

    Present address: Department of Neurology, 200 Hawkins Drive, University of Iowa Hospitals and Clinics, Iowa City, IA, 52242, USA

  3. Mohd. Moshahid Khan

    Present address: Department of Neurology, Medical Research Centre, University of Iowa, Iowa City, IA, 52246, USA

  4. M. Badruzzaman Khan

    Present address: Center for Molecular Chaperone, 1410 Laney Walker Blvd. CN3141/21A, Medical College of Georgia, Augusta, GA, 30912, USA

  5. Pallavi Shrivastava

    Present address: Department of Neurology, UMDNJ, Robert Wood Johanson Medical School, Brunswick, NJ, USA

Authors and Affiliations

  1. Neurotoxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India

    Andleeb Khan, Kumar Vaibhav, Hayate Javed, Rizwana Tabassum, Md. Ejaz Ahmed, Mohd. Moshahid Khan, M. Badruzzaman Khan, Pallavi Shrivastava, M. Saeed Siddiqui & Fakhrul Islam

  2. Department of Biotechnology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India

    Farah Islam

  3. Neuroscience and Toxicology Unit, Faculty of Pharmacy, Jazan University, Jazan, Kingdom of Saudi Arabia

    M. M. Safhi & Fakhrul Islam

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  1. Andleeb Khan
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Correspondence to Fakhrul Islam.

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Khan, A., Vaibhav, K., Javed, H. et al. 1,8-Cineole (Eucalyptol) Mitigates Inflammation in Amyloid Beta Toxicated PC12 Cells: Relevance to Alzheimer’s Disease. Neurochem Res 39, 344–352 (2014). https://doi.org/10.1007/s11064-013-1231-9

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  • DOI: https://doi.org/10.1007/s11064-013-1231-9

Keywords

  • Cineole
  • Amyloid beta (Aβ25–35)
  • NOS-2
  • COX-2
  • NF-κB
  • Cytokines