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Bilateral Common Carotid Artery Ligation Transiently Changes Brain Lipid Metabolism in Rats

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Abstract

Brain lipid metabolism was studied in rats following permanent bilateral common carotid artery ligation (BCCL), a model for chronic cerebral hypoperfusion. Unesterified (free) fatty acids (uFA) and acyl-CoA concentrations were measured 6 h, 24 h, and 7 days after BCCL or sham surgery, in high energy-microwaved brain. In BCCL compared to sham rats, cytosolic phospholipase A2 (cPLA2) immunoreactivity in piriform cortex, and concentrations of total uFA and arachidonoyl-CoA, an intermediate for arachidonic acid reincorporation into phospholipids, were increased only at 6 h. At 24 h, immunoreactivity for secretory phospholipase A2 (sPLA2), which may regulate blood flow, was increased near cortical and hippocampal blood vessels. BCCL did not affect levels of brain IB4+ microglia, glial fibrillary acidic protein (GFAP)+ astrocytes, cyclooxygenase-2 (COX-2) immunoreactivity at any time, but increased cPLA2 immunoreactivity in one region at 6 h. Thus, BCCL affected brain lipid metabolism transiently, likely because of compensatory sPLA2-mediated vasodilation, without producing evidence of neuroinflammation.

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Abbreviations

ARA:

Arachidonic acid

BCCL:

Bilateral common carotid artery ligation

cPLA2 :

Cytoplasmic phospholipase A2

sPLA2 :

Secretory phospholipase A2

CBF:

Cerebral blood flow

COX-2:

Cyclooxygenase 2

HRP:

Horseradish peroxidase

IB4 :

Isolectin B4

GFAP:

Glial fibrillary acidic protein

uFA:

Unesterified fatty acid

PGE2 :

Prostaglandin E2

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Acknowledgments

This research was supported entirely by the Intramural Research Program of the National Institute on Aging, and by the National Institute of Environmental Health Science, NIH.

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Author notes

  1. Abesh Kumar Bhattacharjee

    Present address: Psychiatry Residency Program, Department of Psychiatry, U.C. San Diego, 9500 Gillman Drive #9116A, La Jolla, CA, 92093, USA

Authors and Affiliations

  1. Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA

    Abesh Kumar Bhattacharjee, Laura White, Lisa Chang, Kaizong Ma, Joseph Deutsch & Stanley I. Rapoport

  2. Neurotoxicology Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA

    G. Jean Harry

  3. School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel

    Joseph Deutsch

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  1. Abesh Kumar Bhattacharjee
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Correspondence to Stanley I. Rapoport.

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Bhattacharjee, A.K., White, L., Chang, L. et al. Bilateral Common Carotid Artery Ligation Transiently Changes Brain Lipid Metabolism in Rats. Neurochem Res 37, 1490–1498 (2012). https://doi.org/10.1007/s11064-012-0740-2

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