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Structural Characterization of Monosialo-, Disialo- and Trisialo-gangliosides by Negative Ion AP-MALDI-QIT-TOF Mass Spectrometry with MSn Switching

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Abstract

The atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) is a quite convenient soft ionization for biomolecules, keeping analytes atmospheric conditions instead of high vacuum conditions. In this study, an AP-MALDI ion source has been coupled to a quadrupole ion trap time-of-flight (QIT-TOF) mass spectrometer, which is able to perform MSn analysis. We applied this system to the structural characterization of monosialogangliosides, GM1 (NeuAc) and GM2 (NeuAc), disialogangliosides, GD2 (NeuAc, NeuAc), GD1a (NeuAc, NeuAc) and GD1b (NeuAc, NeuAc) and trisialoganglioside GT1a (NeuAc, NeuAc, NeuAc). In this system, the negative ion mass spectra of MS, MS2 and MS3, a set of three mass spectra, were able to measure within 2 s per cycle. Thus, obtained results demonstrate that the negative ion mode MS, MS2 and MS3 spectra provided sufficient information for the determination of molecular weights, oligosaccharide sequences and ceramide structures, and indicate that the AP-MALDI-QIT-TOF mass spectrometry keeping analytes atmospheric conditions with MSn switching is quite useful and convenient for structural analyses of various types of sialic acid-containing GSLs, gangliosides.

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Abbreviations

AP:

Atmospheric pressure

MALDI:

Matrix-assisted laser desorption/ionization

QIT-TOF MS:

Quadrupole ion trap time-of-flight mass spectrometry

GSLs:

Glycosphingolipids

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Acknowledgments

This work was supported by a grant for Supporting Research Centers in Private Universities and by Grant-in-Aid for Scientific Research (A).

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Authors and Affiliations

  1. Disease Glycomics Team, Systems Glycobiology Research Group, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan

    Emi Ito & Naoyuki Taniguchi

  2. Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto, 604-8511, Japan

    Akio Tominaga, Hiroaki Waki, Kozo Miseki & Minoru Suzuki

  3. Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), 2-12 Tsukuba Central, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan

    Azusa Tomioka

  4. Glycan Recognition Team, Systems Glycobiology Research Group, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan

    Kazuki Nakajima & Naoyuki Taniguchi

  5. School of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashi-Osaka, 577-8502, Japan

    Kazuaki Kakehi

  6. Institute of Glycoscience, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa, 259-1292, Japan

    Akemi Suzuki

Authors
  1. Emi Ito
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  2. Akio Tominaga
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  3. Hiroaki Waki
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  7. Kazuaki Kakehi
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  8. Minoru Suzuki
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  9. Naoyuki Taniguchi
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  10. Akemi Suzuki
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Corresponding author

Correspondence to Akemi Suzuki.

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Special Issue: In honor of Bob Leedeen.

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Ito, E., Tominaga, A., Waki, H. et al. Structural Characterization of Monosialo-, Disialo- and Trisialo-gangliosides by Negative Ion AP-MALDI-QIT-TOF Mass Spectrometry with MSn Switching. Neurochem Res 37, 1315–1324 (2012). https://doi.org/10.1007/s11064-012-0735-z

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  • DOI: https://doi.org/10.1007/s11064-012-0735-z

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