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Expression of the Brain and Muscle Isoforms of Glycogen Phosphorylase in Rat Heart

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Abstract

Heart glycogen represents a store of glucosyl residues which are mobilized by the catalysis of glycogen phosphorylase (GP) and are mainly destined to serve as substrates for the generation of ATP. The brain isoform of GP (GP BB) was studied in rat heart in comparison with the muscle isoform (GP MM) to find functional analogies to the brain. Western blotting and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) experiments revealed that at the protein level, but not at the mRNA level, the content of GP BB is similar in heart and brain. In contrast, GP MM is more abundant in the heart than in the brain. Immunocytochemically GP BB was colocalized with GP MM in cardiomyocytes. GP MM was also detected in interstitial cells identified as fibroblasts. The physiological role of co-expression of GP BB and GP MM in cardiomyocytes and in brain astrocytes is discussed in a comparative way.

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References

  1. Fletterick RJ, Burke JA, Hwang HP et al (1986) Structural relationships in glycogen phosphorylases. Ann NY Acad Sci 478:220–232. doi:10.1111/j.1749-6632.1986.tb15533.x

    Article  PubMed  CAS  Google Scholar 

  2. Newgard CB, Hwang PK, Fletterick RJ (1989) The family of glycogen phosphorylases: structure and function. Crit Rev Biochem Mol Biol 24:69–99. doi:10.3109/10409238909082552

    Article  PubMed  CAS  Google Scholar 

  3. Crerar MM, Karlsson O, Fletterick RJ et al (1995) Chimeric muscle and brain glycogen phosphorylases define protein domains governing isozyme-specific responses to allosteric activation. J Biol Chem 270:13748–13756. doi:10.1074/jbc.270.23.13748

    Article  PubMed  CAS  Google Scholar 

  4. Pfeiffer-Guglielmi B, Bröer S, Bröer A et al (2000) Isozyme pattern of glycogen phosphorylase in the rat nervous system and rat astroglia-rich primary cultures: electrophoretic and polymerase chain reaction studies. Neurochem Res 5:1485–1491. doi:10.1023/A:1007676109206

    Article  Google Scholar 

  5. Pfeiffer-Guglielmi B, Fleckenstein B, Jung G et al (2003) Immunocytochemical localization of glycogen phosphorylase isozymes in rat nervous tissues by using isozyme-specific antibodies. J Neurochem 85:73–81

    Article  PubMed  CAS  Google Scholar 

  6. Pfeiffer-Guglielmi B, Francke M, Reichenbach A et al (2007) Glycogen phosphorylase isozymes and energy metabolism in the rat peripheral nervous system—an immunocytochemical study. Brain Res 1136:20–27. doi:10.1016/j.brainres.2006.12.037

    Article  PubMed  CAS  Google Scholar 

  7. Kato K, Shimizu A, Kurobe N et al (1989) Human brain-type glycogen phosphorylase: quantitative localization in human tissues determined with an immunoassay system. J Neurochem 52:1425–1432. doi:10.1111/j.1471-4159.1989.tb09189.x

    Article  PubMed  CAS  Google Scholar 

  8. Krause EG, Rabitzsch G, Noll F et al (1996) Glycogen phosphorylase isoenzyme BB in diagnosis of myocardial ischaemic injury and infarction. Mol Cell Biochem 160–161:289–295. doi:10.1007/BF00240061

    Article  PubMed  Google Scholar 

  9. Mair J (1998) Glycogen phosphorylase isoenzyme BB to diagnose ischaemic myocardial damage. Clin Chim Acta 272:79–86. doi:10.1016/S0009-8981(97)00254-4

    Article  PubMed  CAS  Google Scholar 

  10. Peetz D, Post F, Schinzel H et al (2005) Glycogen phosphorylase BB in acute coronary syndromes. Clin Chem Lab Med 43:1351–1358. doi:10.1515/CCLM.2005.231

    Article  PubMed  CAS  Google Scholar 

  11. Richter F, Böhme H-J, Hofmann E (1983) Developmental changes of glycogen phosphorylase b isozymes in rat tissues. Biomed Biochim Acta 42:1229–1235

    PubMed  CAS  Google Scholar 

  12. Richter F, Böhme H-J, Hofmann E (1988) Changes of glycogen phosphorylase isozyme pattern, in rat tissues during pre- and postnatal development. Biomed Biochim Acta 47:743–752

    PubMed  CAS  Google Scholar 

  13. Rannels SR, Lin L, Weaver TE (1997) Expression of glycogen phosphorylase isozymes in developing rat lung. Am J Physiol 273:L389–L394

    PubMed  CAS  Google Scholar 

  14. Hashimoto K, Tamura K, Otani H et al (1995) Histocytochemical and immunohistochemical studies related to the role of glycogen in human developing digestive organs. Anat Embryol (Berl) 192:497–505. doi:10.1007/BF00187180

    CAS  Google Scholar 

  15. Sato K, Satoh K, Sato T et al (1976) Isozyme patterns of glycogen phosphorylase in rat tissues and transplantable hepatomas. Cancer Res 36:487–495

    PubMed  CAS  Google Scholar 

  16. David ES, Crerar MM (1986) Quantitation of muscle phosphorylase mRNA and enzyme amounts in adult tissues. Biochim Biophys Acta 880:78–90

    PubMed  CAS  Google Scholar 

  17. Schmid H, Dolderer B, Thiess U et al (2008) Renal expression of the brain and muscle isoforms of glycogen phosphorylase in different cell types. Neurochem Res (Epub Mar 13). doi:10.1007/s11064-008-9640-x

  18. Mlodzik K, Loffing J, Le Hir M et al (1995) Ecto-5′-nucleotidase is expressed by pericytes and fibroblasts in the rat heart. Histochem Cell Biol 103:227–236. doi:10.1007/BF01454028

    Article  PubMed  CAS  Google Scholar 

  19. Robinson MM (1987) Fixation and immunofluorescent analysis of creatine kinase isozymes in embryonic skeletal muscle. J Histochem Cytochem 35:717–722

    PubMed  CAS  Google Scholar 

  20. Gossrau R (1971) Histochemical, fluorescence microscopical and experimental investigations on the impulse conducting system of golden hamsters, mice and rats. Histochemie 26:44–60. doi:10.1007/BF00307783

    Article  PubMed  CAS  Google Scholar 

  21. Pathak CL, Goyal S (1978) Phosphorylase activity as a histochemical marker of specialized tissue of heart. Histochem J 10:633–640. doi:10.1007/BF01003114

    Article  PubMed  CAS  Google Scholar 

  22. De Tata V, Bergamini C, Gori Z et al (1983) Transmural gradient of glycogen metabolism in the normal rat left ventricle. Pflugers Arch 396:60–65. doi:10.1007/BF00584699

    Article  PubMed  Google Scholar 

  23. Henry CG, Lowry OH (1985) Enzymes and metabolites of glycogen metabolism in canine cardiac Purkinje fibers. Am J Physiol 248:H599–H605

    PubMed  CAS  Google Scholar 

  24. Gabrielli F, Aita M, Arturi E et al (1992) A comparative enzyme histochemical study of glucose metabolism in the conduction system of mammalian hearts. Cell Mol Biol 38:449–455

    PubMed  CAS  Google Scholar 

  25. Slezak J, Tribulova N, Ravingerova T et al (1992) Myocardial heterogeneity and regional variations in response to injury. Lab Invest 67:322–330

    PubMed  CAS  Google Scholar 

  26. Vigoda A, Mamedova LK, Shneyvays V et al (2003) Glycogen metabolism in rat heart muscle cultures after hypoxia. Mol Cell Biochem 254:311–318. doi:10.1023/A:1027302021428

    Article  PubMed  CAS  Google Scholar 

  27. Taegtmeyer H (2004) Glycogen in the heart—an expanded view. J Mol Cell Cardiol 37:7–10. doi:10.1016/j.yjmcc.2004.05.001

    Article  PubMed  CAS  Google Scholar 

  28. Byard RW, Lach B, Preston DN (1991) Peripheral nerve and vasculature involvement in myophosphorylase deficiency (McArdles’s disease). Pathology 23:62–65. doi:10.3109/00313029109061442

    Article  PubMed  CAS  Google Scholar 

  29. Miranda AF, Nette EG, Hartlage PL et al (1979) Phosphorylase isoenzymes in normal and myophosphorylase-deficient human heart. Neurology 29:1538–1541

    PubMed  CAS  Google Scholar 

  30. Pfeiffer-Guglielmi B, Francke M, Reichenbach A et al (2005) Glycogen phosphorylase isozyme pattern in mammalian retinal Müller (glial) cells and in astrocytes of retina and optic nerve. Glia 49:84–95. doi:10.1002/glia.20102

    Article  PubMed  Google Scholar 

  31. Pfeiffer-Guglielmi B, Coles JA, Francke M et al (2006) Immunocytochemical analysis of rat vagus nerve by antibodies against glycogen phosphorylase isozymes. Brain Res 1110:23–29. doi:10.1016/j.brainres.2006.06.080

    Article  PubMed  CAS  Google Scholar 

  32. Yunis AA, Fischer EH, Krebs EG (1962) Comparative studies on glycogen phosphorylase, IV Purification and properties of rabbit heart phosphorylase. J Biol Chem 237:2809–2815

    PubMed  CAS  Google Scholar 

  33. Shulman RG, Rothman DL (2001) The “glycogen shunt” in exercising muscle: a role for glycogen in muscle energetics and fatigue. Proc Natl Acad Sci USA 98:457–461. doi:10.1073/pnas.98.2.457

    Article  PubMed  CAS  Google Scholar 

  34. Gutierrez-Correa J, Hod M, Passonneau JV et al (1991) Glycogen and enzymes of glycogen metabolism in rat embryos and fetal organs. Biol Neonate 59:294–302

    Article  PubMed  CAS  Google Scholar 

  35. Crook ED, Crenshaw G, Veerababu G et al (2000) Overexpression of glutamine:fructose-6-phosphate amidotransferase in rat-1 fibroblasts enhances glucose-mediated glycogen accumulation via suppression of glycogen phosphorylase activity. Endocrinology 141:1962–1970. doi:10.1210/en.141.6.1962

    Article  PubMed  CAS  Google Scholar 

  36. Shang J, Lehrman MA (2004) Activation of glycogen phosphorylase with 5-aminoimidazole-4-carboxamide riboside (AICAR), Assessment of glycogen as a precursor of mannosyl residues in glycoconjugates. J Biol Chem 26:12076–12080

    Google Scholar 

  37. Hamprecht B, Dringen R (1995) Energy metabolism. In: Kettenmann H, Ransom B (eds) Neuroglial Cells. Oxford University Press, New York, pp 473–487

    Google Scholar 

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Acknowledgments

We thank Professor Brigitte Kaissling for her generous gift of anti-5′-ectonucleotidase and Ms. Barbara Birk for valuable technical advice in part of this work.

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Authors and Affiliations

  1. Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, 72076, Tuebingen, Germany

    Heide Schmid, Brigitte Pfeiffer-Guglielmi, Benedikt Dolderer, Ulrike Thiess, Stephan Verleysdonk & Bernd Hamprecht

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  1. Heide Schmid
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  2. Brigitte Pfeiffer-Guglielmi
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  3. Benedikt Dolderer
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  4. Ulrike Thiess
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  6. Bernd Hamprecht
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Correspondence to Bernd Hamprecht.

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This article is dedicated to Dr. Ricardo Tapia.

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Schmid, H., Pfeiffer-Guglielmi, B., Dolderer, B. et al. Expression of the Brain and Muscle Isoforms of Glycogen Phosphorylase in Rat Heart. Neurochem Res 34, 581–586 (2009). https://doi.org/10.1007/s11064-008-9825-3

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  • DOI: https://doi.org/10.1007/s11064-008-9825-3

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