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Effects of Aβ1–42 on the Subunits of KATP Expression in Cultured Primary Rat Basal Forebrain Neurons

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Abstract

ATP-sensitive potassium channels (KATP) play a crucial role in coupling metabolic energy to the membrane potential of cells, thereby functioning as cellular “metabolic sensors.” Recent evidence has showed a connection between the amyloid neurotoxic cascade and metabolic impairment. With regard to their neuroprotection in other neuronal preparations, KATP channels may mediate a potential neuroprotective role in Alzheimer’s disease (AD). To investigate the effects of Aβ1–42 on the subunits of KATP expression in cultured primary rat basal forebrain cholinergic neurons, primary rat basal forebrain neurons were cultured and evaluated. The subunits of KATP: Kir6.1, Kir6.2, SUR1 and SUR2 expressing changes were observed by double immunofluorescence and immunoblotting when the neurons were exposed to Aβ1–42(2 μM) for different time (0, 24, 72 h). We found a significant increase in the expression of Kir6.1 and SUR2 in the cultured neurons being exposed to Aβ1–42 for 24 h, while Kir6.2 and SUR1 showed no significant change. However, after being treated with Aβ1–42 for 72 h, the expression of the four subunits was all increased significantly compared with the control. These findings suggest that being exposed to Aβ1–42 for different time (24 and 72 h) induces differential regulations of KATP subunits expression in cultured primary rat basal forebrain cholinergic neurons. The change in composition of KATP may contribute to resist the toxicity of Aβ1–42.

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Acknowledgments

This work was supported by the Young Scholars General Program of National Natural Science Foundation of the People’s Republic of China (Project proposal No. 30600202); the International Exchange and Cooperation Program of National Natural Science Foundation of the People’s Republic of China (No. 30710303072).

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Authors and Affiliations

  1. Department of Neurology, Provincial Hospital affiliated to Shandong University, No. 324 Jingwu Weiqi Road, Jinan, 250021, Shandong, P.R. China

    Guozhao Ma, Qingxi Fu, Yong Zhang, Yifeng Du, Chunfu Chen, Yuanxiao Cui & Lin lu

  2. Department of ICU, Provincial Hospital affiliated to Shandong University, No. 324 Jingwu Weiqi Road, Jinan, 250021, Shandong, P.R. China

    Jinjiao Jiang

  3. Department of Physiology, School of Medicine, Shandong University, No. 44 Western Wenhua Road, Jinan, 250012, Shandong, P.R. China

    Jianxin Gao, Ailing Bi & Kejing Liu

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  1. Guozhao Ma
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  2. Qingxi Fu
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  3. Yong Zhang
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  9. Chunfu Chen
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  10. Yuanxiao Cui
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Correspondence to Guozhao Ma or Yong Zhang.

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Guozhao Ma and Qingxi Fu are contributed equally to this work.

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Ma, G., Fu, Q., Zhang, Y. et al. Effects of Aβ1–42 on the Subunits of KATP Expression in Cultured Primary Rat Basal Forebrain Neurons. Neurochem Res 33, 1419–1424 (2008). https://doi.org/10.1007/s11064-008-9603-2

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  • DOI: https://doi.org/10.1007/s11064-008-9603-2

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