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The Changes of Cytoskeletal Proteins in Plasma of Acrylamide-Induced Rats

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Abstract

Acrylamide (ACR) is a known industrial neurotoxic chemical that can induce neurodegeneration. Cytoskeletal protein aggregation is a pathological hallmark of neurodegenerative disorders. This study was an initial exploration on cytoskeletal proteins in plasma as potential biomarkers of ACR neurotoxicity. Low and high ACR groups received 20 mg/kg and 40 mg/kg ACR by intraperitoneal injection in adult Wistar rats and control group received physiological saline. Rats were all killed after 8 weeks to evaluate the levels of neurofilament(NF)-L, NF-M, NF-H, β-actin, α-tubulin, β-tubulin, tau, MAP2 proteins in plasma using both SDS-PAGE and western blotting. Compared with the control, the levels of NF-L, NF-M, NF-H, β-actin, tau, MAP2 proteins decreased and the level of α-tubulin increased in high ACR group, the levels of α-tubulin, β-tubulin and MAP2 increased in low ACR group. The results suggested that the changes of these proteins might be relevant to the neurotoxicity of ACR. Some of the cytoskeletal proteins in plasma might be used as marker of biological effect in ACR induced neuropathy.

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Acknowledgment

This work was supported by grants from the Ministry of Science and Technology of China (No. 2002CB512907), and National Natural Science Fund of China (No. 30271138), and Young Excellence Fund of Shandong Province, China (No. 02B5080).

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Authors and Affiliations

  1. Institute of Occupational Health and Environmental Health, Shandong University, Jinan, 250012, P.R.China

    Chao Yi, Guozhen Li & Sufang Yu

  2. Institute of Toxicology, Shandong University, 44 Wenhua Xi Road, Jinan, China, Jinan, Shandong, 250012, P.R.China

    Keqin Xie, Fuyong Song, Lihua Yu, Xiulan Zhao & Sufang Yu

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  1. Chao Yi
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  2. Keqin Xie
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  3. Fuyong Song
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  6. Guozhen Li
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Correspondence to Sufang Yu.

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Yi, C., Xie, K., Song, F. et al. The Changes of Cytoskeletal Proteins in Plasma of Acrylamide-Induced Rats. Neurochem Res 31, 751–757 (2006). https://doi.org/10.1007/s11064-006-9079-x

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