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Decreased α1-Adrenergic Receptor Binding in the Cerebral Cortex and Brain Stem during Pancreatic Regeneration in Rats

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Abstract

The purpose of this study was to investigate the role of brain α1-adrenergic receptor binding in the rat model of pancreatic regeneration using 60–70% pancreatectomy. The α1-adrenergic receptors kinetics was studied in the cerebral cortex and brain stem of sham operated, 72 h pancreatectomised and 7 days pancreatectomised rats. Scatchard analysis with [3H]prazosin in cerebral cortex and brain stem showed a significant decrease (P < 0.01), (P < 0.05) in maximal binding (B max) with a significant decrease (P < 0.001), (P < 0.01) in the K d in 72 h pancreatectomised rats compared with sham respectively. Competition analysis in cerebral cortex and brain stem showed a shift in affinity during pancreatic regeneration. The sympathetic activity was decreased as indicated by the significantly decreased norepinephrine level in the plasma (P < 0.001), cerebral cortex (P < 0.01) and brain stem (P < 0.001) of 72 h pancreatectomised rats compared to sham. Thus, from our results it is suggested that the central α1-adrenergic receptors have a functional role in the pancreatic regeneration mediated through the sympathetic pathway.

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Acknowledgements

This work was supported by a research grant from Department of Biotechnology, Govt. of India. to Dr. C. S. Paulose and Ani Das thanks CSIR for the financial assistance.

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  1. Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, 682 022, Kerala, India

    V. Ani Das, B. Savitha & C. S. Paulose

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  1. V. Ani Das
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  2. B. Savitha
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  3. C. S. Paulose
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Correspondence to C. S. Paulose.

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Ani Das, V., Savitha, B. & Paulose, C.S. Decreased α1-Adrenergic Receptor Binding in the Cerebral Cortex and Brain Stem during Pancreatic Regeneration in Rats. Neurochem Res 31, 727–734 (2006). https://doi.org/10.1007/s11064-006-9073-3

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