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Decreased 5-HT1A Receptor Gene Expression and 5-HT1A Receptor Protein in the Cerebral Cortex and Brain Stem during Pancreatic Regeneration in Rats

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Abstract

The present study was to investigate the role of central 5-HT and 5-HT1A receptor binding and gene expression in a rat model of pancreatic regeneration using 60% pancreatectomy. The pancreatic regeneration was evaluated by 5-HT content and 5-HT1A receptor gene expression in the cerebral cortex (CC) and brain stem (BS) of sham operated, 72 h and 7 days pancreatectomised rats. 5-HT content significantly increased in the CC (P < 0.01) and BS (P < 0.05) of 72 h pancreatectomised rats. Sympathetic activity was decreased as indicated by the significantly decreased norepinephrine (NE) and epinephrine (EPI) level (P < 0.001 and P < 0.05) in the plasma of 72 h pancreatectomised rats. 5-HT1A receptor density and affinity was decreased in the CC (P < 0.01) and BS (P < 0.01). These changes correlated with a diminished 5-HT1Areceptor mRNA expression in the brain regions studied. Our results suggest that the brain 5-HT through 5-HT1A receptor has a functional role in the pancreatic regeneration through the sympathetic regulation.

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  1. Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, 682 022, India

    Valiya veettil Mohanan, S. Balarama Kaimal & Cheramadathikudyil S. Paulose

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  1. Valiya veettil Mohanan
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  2. S. Balarama Kaimal
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  3. Cheramadathikudyil S. Paulose
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Correspondence to Cheramadathikudyil S. Paulose.

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Mohanan, V.v., Kaimal, S.B. & Paulose, C.S. Decreased 5-HT1A Receptor Gene Expression and 5-HT1A Receptor Protein in the Cerebral Cortex and Brain Stem during Pancreatic Regeneration in Rats. Neurochem Res 30, 25–32 (2005). https://doi.org/10.1007/s11064-004-9682-7

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