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Structural/Functional Characteristics of Organotypic Spinal Cord Slices under Conditions of Long-Lasting Culturing

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Neurophysiology Aims and scope

Organotypic cultures of the spinal cord possess significant advantages, as compared with routine cell systems in vitro; the cytoarchitectonics, cytospecificity of the cells, cell-to-cell connections, and other characteristics of spinal cord tissues are preserved to a considerable extent. We analyzed structural/functional characteristics of organotypic spinal cord slices of mice under conditions of long-lasting (one to three weeks) culturing. Immunohistochemical staining and patch-clamp recordings from cells of the substantia gelatinosa showed that the morphology of different cell types and cell-to-cell connections typical of normal spinal nerve tissue are clearly manifested in such organotypic spinal cord cultures. This is why that such a technique of spinal cord culturing can be successfully used in subsequent experimental studies, in particular, in the examination of different-type damages of the spinal cord.

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Authors and Affiliations

  1. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine

    O. A. Rybachuk, Yu. A. Lazarenko, V. V. Krotov & N. V. Voitenko

  2. Institute of Genetic and Regenerative Medicine, National Acad. Med. Sci., Kyiv, Ukraine

    O. A. Rybachuk

  3. National University of Kyiv-Mohyla Academy (NaUKMA), Kyiv, Ukraine

    Yu. A. Lazarenko

Authors
  1. O. A. Rybachuk
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  2. Yu. A. Lazarenko
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  3. V. V. Krotov
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  4. N. V. Voitenko
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Correspondence to O. A. Rybachuk.

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Rybachuk, O.A., Lazarenko, Y.A., Krotov, V.V. et al. Structural/Functional Characteristics of Organotypic Spinal Cord Slices under Conditions of Long-Lasting Culturing. Neurophysiology 49, 162–164 (2017). https://doi.org/10.1007/s11062-017-9647-5

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  • DOI: https://doi.org/10.1007/s11062-017-9647-5

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