Inositol 1,4,5-trisphosphate receptors (IP3Rs) play a key role in intracellular calcium signaling. Up to the present time, the question on the existence of only one level of the unitary conductance of such receptors or a few levels of such conductance remained open. In experiments on the isolated nuclei of Purkinje cerebellar neurons of rats, we examined changes in the conductance of channels of these receptors localized on the internal membrane of the nuclear envelope, which were related to voltage variations. In all cases, these channels demonstrated only one level of the unitary conductance; no sublevels were found within a –100 mV to 100 mV range. Suppression of activity of IP3Rs at negative potentials is determined by a decrease in the probability of the open state of the channel. Thus, a hypothesis on the existence of a few levels of the IP3R channel conductance in the examined object has not been confirmed; the release of Ca2+ through channels of these receptors demonstrates a quantum nature.
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Fedorenko, Y.A. Conductance of Channels of IP3 Receptors of the Nuclear Envelope in Purkinje Neurons. Neurophysiology 48, 93–96 (2016). https://doi.org/10.1007/s11062-016-9573-y
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DOI: https://doi.org/10.1007/s11062-016-9573-y