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Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas

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Abstract

Meningiomas, the most frequent benign intracranial and intraspinal types of tumors are normally removed by surgery. Complications can occur when the tumor is critically localized and cannot be completely removed or when comorbidities of the mostly elder patients increase the general surgical risk. Thus, alternate medical treatment concepts for the therapy of meningiomas would be desirable. Curcumin, the active ingredient of the spice plant Curcuma longa has shown anti-tumorigenic actions in many different types of tumors and therefore, its effect on growth and apoptosis of meningioma cells was studied in the present paper. In vitro, treatment of the human Ben-Men-1 meningioma cell line and of a series of 21 primary human meningioma cell cultures with curcumin (1–20 μM) strongly reduced the proliferation in all cases in a dose dependent manner. Cell cycle analysis by fluorescence-activated cell sorting showed growth arrest at G2/M phase, which was confirmed by demonstrating the corresponding modulation of proteins involved in G2/M arrest by immunoblotting and/or confocal laser microscopy. High dosages (20, 50 μM) of curcumin induced a significant increase of apoptosis in Ben-Men-1 and primary meningioma cell cultures as demonstrated by morphological changes of cell nuclei, DNA fragmentation, translocation of cell membrane associated phosphatidyl serine and the induction of apoptotic-acting cleaved caspase-3. Our results suggest that the multi-targeting drug curcumin has potent anti-tumorigenic actions in meningioma cells and might therefore be a putative candidate for the pharmacological treatment of meningiomas.

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Acknowledgments

This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector. We gratefully acknowledge proofreading of the paper by Kristin Lucia.

Conflicts of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Max Planck Institute of Psychiatry, Clinical Neuroendocrinology Group, Kraepelinstr. 10, 80804, Munich, Germany

    S. Curic, Y. Wu, B. Shan, C. Schaaf, D. Utpadel, G. K. Stalla & U. Renner

  2. Neurosurgical Department of the Clinic, Villingen-Schwenningen, Villingen-Schwenningen, Germany

    M. Lange

  3. Neurosurgical Clinic, Technical University Munich, Munichm, Germany

    D. Kuhlen

  4. IBioBA-CONICET-Max Planck Partner Institute, Buenos Aires, Argentina

    M. J. Perone & E. Arzt

  5. Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

    E. Arzt

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  1. S. Curic
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  2. Y. Wu
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  3. B. Shan
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  4. C. Schaaf
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  6. M. Lange
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  11. U. Renner
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Correspondence to U. Renner.

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S. Curic, Y. Wu are joint first authors.

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Curic, S., Wu, Y., Shan, B. et al. Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas. J Neurooncol 113, 385–396 (2013). https://doi.org/10.1007/s11060-013-1148-9

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  • DOI: https://doi.org/10.1007/s11060-013-1148-9

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