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Down-regulation of miR-106b suppresses the growth of human glioma cells

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Abstract

Recently, many studies have found that the miR-106b ~25 cluster plays an oncogenic role in tumor progression. However, the precise role of each microRNAs (miRNAs) in the cluster is not yet clear. In the present study, we examined the expression of miR-106b in glioma samples and a tissue microarray by real-time PCR and in situ hybridization (ISH), respectively, finding that miR-106b is overexpressed in the majority of gliomas. Meanwhile, the expression of miR-106b was positively correlated with tumor grade (p < 0.05). The transfection of a miR-106b anti-sense oligonucleotide (ASON) into three human glioma cell lines (U251, LN229 and TJ905) suppressed the proliferation of these cells. Moreover, the growth of xenograft tumors in nude mice treated with miR-106b ASON was significantly impaired. A bioinformatics analysis predicted that RBL2 may be the target of miR-106b, and dual-luciferase reporter assays identified RBL2, but not RB1 or RBL1, as a target of miR-106b. These results suggest that miR-106b facilitates glioma cell growth by promoting cell cycle progression through the negative regulation of RBL2.

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Acknowledgments

This work was supported by The China National Natural Scientific Fund (81101915), The Project of Tianjin Applied Basic and Cutting-edge Technological Research (12JCQNJC06900), and The Tianjin Health Department Scientific and Technological Fund (2011KZ109).

Conflict of interest

We confirm that there are no known conflicts of interest associated with this publication and that there has been no significant financial support for this work that could have influenced its outcome. We also confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us.

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Authors and Affiliations

  1. Department of Neurosurgery; Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neuro-repair and Regeneration of the Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of the Nervous System, 154 Anshan Road, Heping District, Tianjin, 300052, People’s Republic of China

    Anling Zhang, Jianwei Hao, Kun Wang, Qiang Huang, Kai Yu, Chunsheng Kang, Guangxiu Wang, Zhifan Jia, Lei Han & Peiyu Pu

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  1. Anling Zhang
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  2. Jianwei Hao
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  3. Kun Wang
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  10. Peiyu Pu
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Correspondence to Peiyu Pu.

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Zhang, A., Hao, J., Wang, K. et al. Down-regulation of miR-106b suppresses the growth of human glioma cells. J Neurooncol 112, 179–189 (2013). https://doi.org/10.1007/s11060-013-1061-2

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  • DOI: https://doi.org/10.1007/s11060-013-1061-2

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