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Adventitious rooting of Eucalyptus globulus × maidennii mini-cuttings derived from mini-stumps grown in sand bed and intermittent flooding trays: a comparative study

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Abstract

Eucalyptus globulus Labill and hybrids thereof have low lignin content, favoring cellulose extraction, but are often recalcitrant to clonal propagation. This work analyzed biochemical and morphological changes during adventitious rooting of mini-cuttings of E. globulus × maidenni obtained from mini-stumps cultured in drip fertigated sand bed or intermittent flooding tray commercial propagation systems. Morphological (% rooting, root number and length, mean rooting time) and biochemical parameters (peroxidase activity, total phenolic content and flavonoid content) were monitored to characterize the rooting phases. All of the rooting parameters were equivalent in both systems, indicating comparable efficiency of both methods in clonal propagation. Kinetic profiles of biochemical parameters were also similar, although the activity of peroxidases was an order of magnitude higher and the phenolic content about three times lower in cuttings derived from intermittent flooding-grown mini-stumps than in those derived from sand bed-grown mini-stumps. Taken together, results suggest that rooting phases were similar in both systems: induction before day 5, formation from day 5 to 15, and elongation from day 15 to 45. These data may contribute to the development of rooting phase-specific mineral nutrient solutions to maximize clonal propagation and plant survival.

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Acknowledgments

The authors thank Aracruz Celulose S.A. for the supply of plant material and for the grant-in-aid of research to carry out this investigation. The Brazilian government agencies CAPES (Federal Agency for Graduate Education) and CNPq (National Council for Scientific and Technological Development) provided graduate, undergraduate and research scholarships.

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Authors and Affiliations

  1. Centro de Biotecnologia–UFRGS (Programa de Pós-Graduação em Biologia Celular e Molecular), C.P. 15005, CEP 91501-970, Porto Alegre, RS, Brazil

    Joséli Schwambach, Carolina Michels Ruedell, Márcia Rodrigues de Almeida & Arthur G. Fett-Neto

  2. Departamento de Botânica, Laboratório de Fisiologia Vegetal, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves, 9500, Prédio 43423-Bloco 4,CEP 91509-900, Porto Alegre, RS, Brazil

    Joséli Schwambach, Carolina Michels Ruedell, Márcia Rodrigues de Almeida & Arthur G. Fett-Neto

  3. Aracruz Celulose S.A., Centro de Pesquisa e Tecnologia, Rua São Geraldo 1800, CEP 92500-000, Guaíba, RS, Brazil

    Ricardo Miguel Penchel & Elias Frank de Araújo

Authors
  1. Joséli Schwambach
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  2. Carolina Michels Ruedell
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  3. Márcia Rodrigues de Almeida
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  4. Ricardo Miguel Penchel
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  5. Elias Frank de Araújo
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  6. Arthur G. Fett-Neto
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Correspondence to Arthur G. Fett-Neto.

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Schwambach, J., Ruedell, C.M., de Almeida, M.R. et al. Adventitious rooting of Eucalyptus globulus × maidennii mini-cuttings derived from mini-stumps grown in sand bed and intermittent flooding trays: a comparative study. New Forests 36, 261–271 (2008). https://doi.org/10.1007/s11056-008-9099-2

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  • DOI: https://doi.org/10.1007/s11056-008-9099-2

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