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SFRP5 prevents memory impairment induced by WNT5A in male Wistar rats

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Abstract

Wingless-type family members (Wnt5a) and secreted frizzled-related protein 5 (SFRP5) play important roles in the central nervous system during development. However, the effects of Wnt5a and SFRP5 on learning and memory in adults remain unknown. Here, we evaluated the effects of Wnt5a and SFRP5 on the consolidation of passive avoidance memory in rats. Forty-two male Wistar rats were divided into six groups including saline, Wnt5a(10ng/mL), SFRP5 (30, 60 and 120 ng/ml), and SFRP5+ Wnt5a groups (n=7). Animals were anaesthetized and cannulated for intra-cerebroventricular (ICV) injection using stereotaxic apparatus. Drugs were injected one hour after the training in step-through passive avoidance learning, and 24 h later, memory retention was evaluated. Data were analyzed by one-way ANOVA and post hoc Tucky tests. Findings showed that injection of Wnt5a in a dose of 10 ng/mL, one hour after training, significantly impaired consolidation of passive avoidance memory (P = 0.01), whereas ICV injection of SFRP5 (60 and 120 ng/ml) (P=0.02) showed a significant facilitatory effect on consolidation of memory compared to the saline receiving group. Also, pretreatment with 60 ng/ml SFRP5, 15 min before WNT5A prevents memory impairment. Our findings suggest that ICV injection of Wnt5a one hour after training impairs consolidation of passive avoidance memory, but pretreatment with SFRP5 significantly prevents the deleterious effect of Wnt5a.

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Data will be made available on reasonable request from the correspondent author.

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Acknowledgements

The authors wish to thank the Cellular & Molecular Research Center staff for providing facilities during this project.

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Authors and Affiliations

  1. Neuroscience Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

    Parvin Babaei, Somayeh Kouhestani & Mahshid BadriKoohi

  2. Cellular & Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

    Parvin Babaei, Somayeh Kouhestani & Mahshid BadriKoohi

  3. Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

    Parvin Babaei, Somayeh Kouhestani & Mahshid BadriKoohi

  4. Department of sports physiology, Faculty of Physical Education and sport sciences, Guilan University, Rasht, Iran

    Arsalan Damirchi

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  1. Parvin Babaei
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Contributions

PB conceived and designed the experiment, contributed to the interpretation of data and wrote the first draft of the manuscript. MB performed the behavioural experiments and contributed to the drafting. AD contributed to analyzing and interpreting data, besides providing the grant for research. SK analyzed data, provided figures for the manuscript, and contributed to drafting. All authors reviewed the manuscript and approved the final manuscript.

Corresponding author

Correspondence to Mahshid BadriKoohi.

Ethics declarations

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

The protocol of this study was confirmed by the ethics committee of Guilan University of Medical Sciences, Rasht, Iran, IR.GUMS.REC.1397.058.

Competing Interests and Funding

Authors declare no competing interest. This study was supported by the Grant from Dr Arsalan Damirchi, the Guilan University.

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Babaei, P., Kouhestani, S., Damirchi, A. et al. SFRP5 prevents memory impairment induced by WNT5A in male Wistar rats. Neurosci Behav Physi 52, 784–790 (2022). https://doi.org/10.1007/s11055-022-01261-z

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  • DOI: https://doi.org/10.1007/s11055-022-01261-z

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