We present here an analysis of the ultrastructural characteristics of synapses in the anterior cortical nucleus of the amygdaloid body of the brain in female WAG/Rij rats (n = 9) with different levels of female sex hormones. The results of this study demonstrated that ovariectomy led to structural changes in synapses and decreases in the total number of synapses as compared with the control group, while hormone replacement therapy led to recovery of synapse structure and number. In terms of the level of dense projections, the control and post-ovariectomy groups were dominated by asymmetrical synapses (52%); in terms of the type of curvature of the plane of the synaptic contact, synapses with positively curved contacts dominated. Replacement therapy was followed by a minor decrease in the number of asymmetrical synapses (47%), most having negatively curved contacts. Complex synaptic contacts of the divergent and convergent types were seen, which is evidence that contacts were in an active functional state. The number of perforated synapses increased significantly from 14.3 to 50%, which may be evidence of estrogen activation of synapses.
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References
N. N. Bogolepov, “Ultrastructure of human cerebral cortex synapses in old age,” Eksper. Fundam. Nevrol., 2, No. 4, 22–27 (2008).
N. N. Bogolepov, “Characteristics of the plasticity and stability of the synaptoarchitectonics of the brain,” Vestn. Mezhdunar. Akad. Nauk, No. 1, 18–21 (2010).
Ya. M. Kabak, Handbook in Endocrinology, Moscow State Univ. Press (1968).
I. I. Sadrtdinova and Z. R. Khismatullina, “Steroid regulation of neuronal excitability in the anterior cortical nucleus of the amygdaloid complex of the brain in WAG/ Rij rats,” Biomeditsina, No. 2, 64–72 (2014).
V. V. Semchenko, N. N. Bogolepov, and S. S. Stepanov, The Synaptoarchitectonics of the Cerebral Cortex (morphometric aspects), IPK Omich, Omsk (1995).
V. V. Semchenko, S. S. Stepanov, and N. N. Bogolepov, Synaptic Plasticity of the Brain (fundamental and applied aspects), Direkt-Media, Moscow (2004).
Z. R. Khismatullina, The Amygdaloid Complex of the Brain in the System Regulating the Reproductive Functions of the Body: Auth. Abstr. Dissert. Doct. Biol. Sci., Ufa (2009).
O. M. Shirokova, M. V. Vedunova, E. V. Mitroshina, et al., “Mor phofunctional features of the development of neural networks in dissociated hippocampal cell cultures,” Sovrem. Tekhnol. Med., 5, No. 2, 6–13 (2013).
S. Matsuda, Y. Kobayashi, and N. Ishizuka, “A quantitative analysis of the laminar distribution of synaptic boutons in field CA3 of the rat hippocampus,” Neurosci. Res., 49, 241–251 (2004).
P. G. Mermelstein, “Membrane-localised oestrogen receptor alpha and beta influence neuronal activity through activation of metabotropic glutamate receptors,” J. Neuroendocrinology, 21, No. 4, 257–262 (2009).
M. Morissette, M. Le Saux, M. D’Astous, et al, “Contribution of estrogen receptors alpha and beta to the effects of estradiol in the brain,” J. Steroid Biochem. Mol. Biol., 108, No. 3, 327–338 (2008).
A. Parducz and M. Garcia-Segura, “Sexual differences in the synaptic connectivity in the rat dentate gyrus,” Neurosci. Lett., 161, 53–56 (1993).
A. Rollenhagen and J. H. Lubke, “The morphology of excitatory central synapses: from structure to function,” Cell Tissue Res., 326, No. 2, 221–237 (2006).
Q. Tai, K. Palazzolo, A. Mautes, et al., “Ultrastructural characteristics of glutamatergic and GABAergic terminals in cat lamina IX before and after spinal cord injury,” J. Spinal Cord Med., 20, No. 3, 311–318 (1997).
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Translated from Morfologiya, Vol. 151, No. 2, pp. 30–34, March–April, 2017.
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Sadrtdinova, I.I., Khismatullina, Z.R. Changes in the Ultrastructure of Synapses in the Anterior Cortical Nucleus of the Amygdaloid Body of the Brain in Female Rats in Relation to Sex Hormone Levels. Neurosci Behav Physi 48, 432–435 (2018). https://doi.org/10.1007/s11055-018-0583-7
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DOI: https://doi.org/10.1007/s11055-018-0583-7