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The Vascular Network and Neuromuscular Synapses in Skeletal Muscle in Combined Chemical Denervation

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The gastrocnemius muscle in white mongrel female rats was studied from postnatal day 14 to postnatal day 180 in normal conditions (control group, n = 18), after neonatal chemical deafferentation (n = 18), after neonatal chemical desympathization (n = 18), and after combined neonatal chemical denervation (deafferentation plus desympathization, n = 18). Controls consisted of intact animals (n = 18). Histochemical methods were used to detect cholinesterase (using thioacetic acid as substrate) and alkaline phosphatase (using naphthol AS-BS as substrate) activities. Combined chemical denervation showed a reduction in cholinesterase activity compared with controls, block of early developmental elimination of neuromuscular synapses, a lower proportion of complex synapse-active zones, and later formation of vascular loops around motor endings. The vascular network had larger-diameter vessels, most with a planar orientation. The forming “muscle fi ber – motor ending – vascular network” system had low adaptive potential and a tendency to earlier development of involutional processes.

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Authors and Affiliations

  1. Department of Human Anatomy and Physiology, Yaroslavl State Pedagogical University, Yaroslavl, Russia

    T. R. Kovrigina

  2. Department of Operative Surgery and Topographical Anatomy, Yaroslavl State Medical Academy, Yaroslavl, Russia

    V. I. Filimonov

Authors
  1. T. R. Kovrigina
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  2. V. I. Filimonov
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Correspondence to T. R. Kovrigina or V. I. Filimonov.

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Translated from Morfologiya, Vol. 146, No. 6, pp. 54–59, November–December, 2014.

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Kovrigina, T.R., Filimonov, V.I. The Vascular Network and Neuromuscular Synapses in Skeletal Muscle in Combined Chemical Denervation. Neurosci Behav Physi 45, 996–1000 (2015). https://doi.org/10.1007/s11055-015-0177-6

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  • DOI: https://doi.org/10.1007/s11055-015-0177-6

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