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Sympathetic Innervation and Endogenous Catecholamines in Neuromuscular Preparations of Muscles with Different Functional Profiles

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Abstract

Influence of the sympathetic nervous system on the work of skeletal muscles contractile apparatus is now beyond doubt. However, until recently there was no evidence that the endings of sympathetic nerves can be located in close proximity to the neuromuscular synapses, and there is also no reliable data on how much endogenous adrenaline and noradrenaline can be contained near the synaptic contact in skeletal muscles. In this research, using fluorescent analysis, immunohistochemical and enzyme immunoassays the isolated neuromuscular preparations of three skeletal muscles of different functional profiles and containing different types of muscle fibers were examined. Close contact between the sympathetic and motor cholinergic nerve endings and the presence of tyrosine hydroxylase in this area were demonstrated. Concentrations of endogenous adrenaline and noradrenaline in the solution perfusing the neuromuscular preparation were determined under different modes of its functioning. The effects of α and β adrenoreceptor blockers on the processes of acetylcholine quantal secretion from the motor nerve endings were compared. The data obtained provide evidence for the presence of endogenous catecholamines in the neuromuscular junction region and their role in modulation of the synaptic function.

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Abbreviations

AD:

adrenaline

ADR:

adrenoreceptors

NA:

noradrenaline

NMP:

neuromuscular preparation

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Acknowledgments

The authors express their gratitude to A.M. Petrov for critical review of the manuscript and helpful suggestions.

Funding

This work was financially supported by the Russian Science Foundation (project no. 18-15-0046). ELISA, sample preparation and data analysis were performed by S.A. Dmitrieva and S.G. Vologin with the support from the government assignment for FRC Kazan Scientific Center of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Federal Research Center “Kazan Scientific Center of the Russian Academy of Sciences”, 420111, Kazan, Russia

    Svetlana A. Dmitrieva, Andrei N. Tsentsevitsky, Arsenii Yu. Arkhipov, Venera F. Khuzakhmetova, Guzel V. Sibgatullina & Ellya A. Bukharaeva

  2. Tatar Research Institute of Agriculture, Federal Research Center “Kazan Scientific Center of the Russian Academy of Sciences”, 420059, Kazan, Russia

    Semyon G. Vologin

Authors
  1. Svetlana A. Dmitrieva
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  2. Semyon G. Vologin
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  3. Andrei N. Tsentsevitsky
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  4. Arsenii Yu. Arkhipov
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  5. Venera F. Khuzakhmetova
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  6. Guzel V. Sibgatullina
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  7. Ellya A. Bukharaeva
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Contributions

E.A. Bukharaeva – conceptualization, supervision, methodology, writing, review & editing; S.A. Dmitrieva, S.G. Vologin – enzyme immunoassay; A.N. Tsentsevitzky, A.Yu. Arkhipov, V.F. Khuzakhmetova – preparing and investigation of experimental samples; G.V. Sibgatullina – immunohistochemical analysis.

Corresponding author

Correspondence to Ellya A. Bukharaeva.

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The authors declare no conflict of interest in financial or any other sphere. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Dmitrieva, S.A., Vologin, S.G., Tsentsevitsky, A.N. et al. Sympathetic Innervation and Endogenous Catecholamines in Neuromuscular Preparations of Muscles with Different Functional Profiles. Biochemistry Moscow 88, 364–373 (2023). https://doi.org/10.1134/S0006297923030069

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