Studies on common snails previously trained to an associative skill consisting of rejecting a defined foodstuff addressed the effects of NMDA glutamate receptor antagonists (MK-801 and APV) and protein synthesis inhibitors (cycloheximide and anisomycin) on long-term memory reconsolidation processes. Injections of each of the study compounds before the reminding procedure 24 h after training were found to lead to impairment of the reproduction of the acquired skill, which lasted at least three weeks. Repeat training of these animals to reject the same foodstuff as used in the initial training did not lead to acquisition of the skill. However, simultaneous injections of a protein synthesis inhibitor and an NMDA receptor antagonist (MK-801 + cycloheximide or APV + anisomycin) did not impair the skill. In subsequent experiments, snails received cycloheximide at different times after exposure to MK-801/reminding. Administration of cycloheximide 3 and 6 h after MK-801/reminding led to the development of incomplete amnesia and repeat training of the animals led to rapid restoration of memory. Administration of cycloheximide 9 h after MK-801/reminding evoked the development of stable amnesia characterized by impairment of skill formation on repeat training. We propose that the mechanisms of amnesia induced by the NMDA glutamate receptor antagonist, by analogy with the mechanisms of other long-term adaptive rearrangements of the brain, depend on translation and can be suppressed by inhibitors of translation. The “time window” for the dependence of amnesia induction processes on the synthesis of protein molecules was 6–9 h after exposure to MK-801/reminding.
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S. V. Solntseva and V. P. Nikitin, “Serotonin and NMDA glutamate receptor antagonists selectively impair the reactivation of associative memory in the common snail,” Ros. Fiziol. Zh. im. I. M. Sechenova, 93, No. 10, 1101–1111 (2007).
S. V. Solntseva and V. P. Nikitin, “Reversible and irreversible stages in the development of amnesia after impairments of the reactivation of associative memory in snails,” Zh. Vyssh. Nerv. Deyat., 59, No. 2, 344–352 (2009).
S. V. Solntseva, V. P. Nikitin, S. A. Kozyrev, A. V. Nikitin, A. V. Lagutin, and V. V. Sherstnev, “Inhibition of protein synthesis during reactivation of associative memory in the common snail evokes transient or irreversible amnesia,” Ros. Fiziol. Zh. im. I. M. Sechenova, 92, No. 9, 1058–1068 (2006).
I. Akirav and M. Maroun, “Ventromedial prefrontal cortex is obligatory for consolidation and reconsolidation of object recognition memory,” Cereb. Cortex, 16, No. 12, 1759–1765 (2006).
K. V. Anokhin, A. A. Tiunova, and S. P. R. Rose, “Reminder effects – reconsolidation or retrieval deficit? Pharmacological dissection with protein synthesis inhibitors following reminder for a passiveavoidance task in young chicks,” Eur. J. Neurosci., 15, No. 11, 1759–1765 (2002).
C. H. Bailey and E. R. Kandel, “Synaptic remodeling, synaptic growth and the storage of long-term memory in Aplysia,” Prog. Brain Res., 169, 179–298 (2008).
P. M. Balaban, “Declarative and procedural memory in animals with simple nervous systems,” in: Psychology at the Turn of the Millennium,” C. Hofsten (ed.), Akad. Press, Stockholm (2002), Vol. 1, pp. 1–28.
A. Citri and R. C. Malenka, “Synaptic plasticity: multiple forms, functions, and mechanisms,” Neuropsychopharmacology Rev., 1–24 (2007).
H. P. Davis and L. R. Squire, “Protein synthesis and memory: a review,” Psychol. Bull., 96, No. 4, 518–559 (1984).
T. H. Gainutdinova, T. T. Tagirova, A. I. Ismailova, L. M. Muranova, E. I. Samarova, K. L. Gainutdinov, and P. M. Balaban, “Reconsolidation of a context long-term memory in the terrestrial snail requires protein synthesis,” Learn. Mem., 12, No. 6, 620–625 (2005).
P. Goelet, V. F. Castellucci, S. Schacher, and E. Kandel, “The long and the short of long-term memory – a molecular framework,” Nature, 322, No. 6078, 419–422 (1986).
L. M. Igaz, M. R. M. Vianna, J. H. Medina, and I. Izquierdo, “Two time periods of hippocampal mRNA synthesis are required for memory consolidation of fear-motivated learning,” J. Neurosci., 22, No. 15, 6781–6789 (2002).
K. M. Lattal and T. Abel, “Behavioral impairments caused by injections of the protein synthesis inhibitor anisomycin after contextual retrieval reverse with time,” Proc. Natl. Acad. Sci. USA, 101, No. 13, 4667–4672 (2004).
J. L. Lee and B. J. Everitt, “Appetitive memory reconsolidation depends upon NMDA receptor-mediated neurotransmission,” Neurobiol. Learn. Mem., 90, No. 1, 147–154 (2008).
J. L. McGaugh, “Memory – a century of consolidation,” Science, 287, No. 5451, 248–251 (2000).
M. C. Miniaci, J.-H. Kim, S. V. Puthanveettil, K. Si, H. Zhu, E. R. Kandel, and C. H. Bailey, “Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-term facilitation in Aplysia,” Neuron, 59, 1024–1036 (2008).
K. Nader, “A single standard for memory; the case for reconsolidation,” Debates Neurosci., 1, No. 1, 2–16 (2007).
K. Parvez, V. Moiseev, and K. Lukowiak, “A context-specific single contingent-reinforcing stimulus boosts intermediate-term memory into long-term memory,” Eur. J. Neurosci., 24, No. 2, 606–616 (2006).
M. E. Pedreira, L. M. Perez-Cuesta, and H. Maldonado, “Reactivation and reconsolidation of long-term memory in the crab Chasmagnathus: protein synthesis requirement and mediation by NMDA-type glutamatergic receptors,” J. Neurosci., 22, No. 18, 8305–8311 (2002).
D. C. Riccio, P. M. Millin, and A. R. Bogart, “Reconsolidation: a brief history, a retrieval view, and some recent issues,” Learn. Mem., 13, No. 5, 536–544 (2006).
T. C. Sacktor, “PKMzeta, LTP maintenance, and the dynamic molecular biology of memory storage,” Prog. Brain Res., 169, 27–40 (2008).
W. S. Sossin, “Molecular memory traces,” Prog. Brain Res., 169, 3–25 (2008).
M. Torras-Garcia, J. Lelong, S. Tronel, and S. J. Sara, “Reconsolidation after remembering an odor-reward association requires NMDA receptors,” Learn. Mem., 12, No. 1, 18–22 (2005).
N. C. Tronson and J. R. Taylor, “Molecular mechanisms of memory reconsolidation,” Nat. Rev. Neurosci., 8, No. 4, 262–275 (2007).
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 96, No. 3, pp. 247–258, March, 2010.
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Solntseva, S.V., Nikitin, B.P. Protein Synthesis Is Required for Induction of Amnesia Elicited by Disruption of the Reconsolidation of Long-Term Memory. Neurosci Behav Physi 41, 654–662 (2011). https://doi.org/10.1007/s11055-011-9469-7
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DOI: https://doi.org/10.1007/s11055-011-9469-7