Calcium-binding protein Iba-1 is currently believed to be identical to AIF-1 protein and is regarded as a selective marker for microglia. However, data on populations of cells able to express Iba-1/AIF-1 are contradictory. The aim of the present work was to identify rat brain cells expressing Iba-1 protein and to describe their functional characteristics. Immunocytochemical and confocal laser microscopy methods were used. Iba-1 protein was seen only in microglial cells, meningeal macrophages, supraependymal macrophages, and superficial and stromal cells of the vascular plexus, i.e., cells with phagocytic functions. Comparison of these data with results from other studies suggests that not all of the currently used antibodies to Iba-1/AIF-1 protein recognize the same product. This may be associated with alternative splicing of the Iba-1 mRNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
N. I. Artyukhina and K. Yu. Sarkisova, “Interhemisphere asymmetry of hippocampal lesions after bilateral ligation of the common carotid arteries,” Ros. Fiziol. Zh., 90, No. 2, 146–156 (2004).
V. K. Beletskii, “Neurology – normal and pathological,” in: A Multivolume Handbook for Pathological Anatomy [in Russian], Gos. Izd. Med. Lit. Press, Moscow (1963), Vol. II, pp. 55–82.
D. E. Korzhevskii, “Tissue organization and development of the choroid plexus of the human brain,” Morfologiya, 113, No. 2, 105–114 (1998).
D. E. Korzhevskii, “Macrophages of the choroid plexus of the hindbrain in the human embryo,” Morfologiya, 119, No. 1, 20–23 (2001).
F. E. Korzhevskii, I. P. Grigoriev, and V. A. Otellin, “Use of dehydrating fixatives containing zinc salts in neurohistological studies,” Morfologiya, 129, No. 1, 85–87 (2006).
L. Broglio, B. Erne, M. Tolnay, et al., “Allograft inflammatory factor-1: a pathogenetic factor for vasculitic neuropathy,” Muscle Nerve, 38, No. 4, 1272–1279 (2008).
M. H. Deininger, R. Meyermann, and H. J. Schluesener, “The allograft inflammatory factor-1 family of proteins,” FEBS Lett., 514, No. 2, 115–121 (2002).
Y. Imai, I. Ibata, D. Ito, et al., “A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage,” Biochem. Biophys. Res. Commun., 224, No. 3, 855–862 (1996).
D. Ito,Y. Imai, K. Ohsawa, et al., “Microglia-specific localization of a novel calcium binding protein, Iba1,” Brain Res. Mol. Brain Res., 57, No. 1, 1–9 (1998).
C. Kohler, “Allograft inflammatory factor-1/Ionized calcium-binding adapter molecule 1 is specifically expressed by most subpopulations of macrophages and spermatids in testis,” Cell Tiss. Res., 33, No. 2, 291–302 (2007).
S. Liu, W. Y. Tan, Q. R. Chen, et al., “Daintain/AIF-1 promotes breast cancer proliferation via activation of the NF-kappa B/cyclin D1 pathway and facilitates tumor growth,” Cancer Sci., 99, No. 5, 952–957 (2008).
J. Mentschel, M. H. Deininger, H. J. Schluesener, and R. Claus, “Effects of malnutrition on the expression of daintain/AIF-1 in the gut mucosa of pigs,” J. Vet. Med. Physiol. Clin. Med., 49, No. 4, 184–188 (2002).
L. J. Sommerville, C. Xing, S. E. Keleman, et al., “Inhibition of allograft inflammatory factor-1 expression reduces development of neointimal hyperplasia and p38 kinase activity,” Cardiovasc. Res., 81, No. 1, 206–215 (2009).
D. Wu, O. Miyamoto, S. Shibuya, et al., “Different expression of macrophages and microglia in rat spinal cord contusion injury model at morphological and regional levels,” Acta Med. Okayama, 59, No. 4, 121–127 (2005).
M. Yamada, K. Ohsawa, Y. Imai, et al., “x-Ray structures of the microglia/macrophage-specific protein Iba-1 from human and mouse demonstrate novel molecular conformation change induced by calcium binding,” J. Mol. Biol., 364, No. 3, 449–457 (2006).
Author information
Authors and Affiliations
Additional information
Translated from Morfologiya, Vol. 137, No. 2, pp. 5–8, March–April, 2010.
Rights and permissions
About this article
Cite this article
Kirik, O.V., Sukhorukova, E.G. & Korzhevskii, D.E. Calcium-Binding Protein Iba-1/AIF-1 in Rat Brain Cells. Neurosci Behav Physi 41, 149–152 (2011). https://doi.org/10.1007/s11055-011-9391-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-011-9391-z