Abstract
The present study was performed to develop a simple and reliable method for the combined staining of specimens to allow the advantages of immunocytochemical detection of astrocytes and assessment of the functional state of neurons by the Nissl method to be assessed simultaneously. The protocol suggested for processing paraffin sections allows preservation of tissue structure at high quality and allows the selective identification of astrocytes with counterstaining of neurons by the Nissl method. The protocol can be used without modification for processing brain specimens from humans and various mammals-except mice and rabbits.
Similar content being viewed by others
REFERENCES
D. E. Korzhevskii, “The use of methylsalicylate as an intermediate medium for embedding in paraffin, ” Morfologiya, 109, No.1, 105 (1996).
D. E. Korzhevskii, “The use of monoclonal antibodies to nuclear protein PCNA for the detection of proliferating cells in the developing human brain,” Morfologiya, 118, No.5, 68–70 (2000).
D. E. Korzhevskii, “Macrophages of the vascular plexus of the end-brain of the human embryo,” Morfologiya, 119, No.1, 20–23 (2001).
A. P. Novozhilova and O. N. Gaikova, “Cellular gliosis of the white matter of the human brain and its importance in the pathogenesis of focal epilepsy,” Morfologiya, 119,, No.2, 20–24 (2001).
V. A. Otellin, D. E. Korzhevskii, V. B. Kostkin, et al., “The neuroprotective effect of creatine in cerebral ischemia,” Dokl. Akad. Nauk SSSR, 390, No.3, 406–408 (2003).
C. Herrington and J. McGee, Molecular Clinical Diagnosis. Methods [Russian translation], Mir, Moscow (1999).
S. Davis, A. Thomas, R. Perry, et al., “Glial fibrillary acidic protein in late life major depressive disorder: an immunocytochemical study,” J. Neurol. Neurosurg. Psychiatry, 77, No.5, 556–560 (2002).
S. J. Naish, Immunochemical Staining Methods, DAKO Corporation (1989).
Author information
Authors and Affiliations
Additional information
Translated from Morfologiya, Vol. 125, No. 3, pp. 100–102, May–June, 2004.
Rights and permissions
About this article
Cite this article
Korzhevskii, D.E., Otellin, V.A. Immunocytochemical Detection of Astrocytes in Brain Slices in Combination with Nissl Staining. Neurosci Behav Physiol 35, 639–641 (2005). https://doi.org/10.1007/s11055-005-0105-2
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11055-005-0105-2