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Photo-crosslinked hyaluronic acid coated upconverting nanoparticles

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Abstract

Hyaluronic acid (HA)-coated inorganic nanoparticles display enhanced interaction with the CD44 receptors which are overexpressed in many types of cancer cells. Here, we describe a modification of core-shell β-NaY0.80Yb0.18Er0.02F4@NaYF4 nanoparticles (UCNP) by HA derivative bearing photo-reactive groups. UCNP capped with oleic acid were firstly transferred to aqueous phase by an improved protocol using hydrochloric acid or lactic acid treatment. Subsequently, HA bearing furanacryloyl moieties (HA-FU) was adsorbed on the nanoparticle surface and crosslinked by UV irradiation. The crosslinking resulted in stable HA coating, and no polymer desorption was observed. As-prepared UCNP@HA-FU show a hydrodynamic diameter of about 180 nm and are colloidally stable in water and cell culture media. The cellular uptake by normal human fibroblasts and MDA MB-231 cancer cell line was investigated by upconversion luminescence imaging.

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Acknowledgments

We would like to thank Hana Vagnerova and Zuzana Tomickova for help with in vitro assays and cell staining; Tomas Bobula for help with UV-crosslinking reactions; Ondrej Zidek and Filip Mika for electron microscopy imaging.

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Authors and Affiliations

  1. Contipro a. s., Dolni Dobrouc 401, 561 02, Dolni Dobrouc, Czech Republic

    Jiri Mrazek, Sofiane Kettou, Vit Matuska, Vit Svozil, Gloria Huerta-Angeles, Martina Pospisilova, Kristina Nesporova & Vladimir Velebny

  2. 3rd Medical Faculty, Department of Medical Biophysics and Medical Informatics, Charles University, Prague, Czech Republic

    Jiri Mrazek

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  1. Jiri Mrazek
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  2. Sofiane Kettou
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  3. Vit Matuska
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  5. Gloria Huerta-Angeles
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  8. Vladimir Velebny
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Correspondence to Jiri Mrazek.

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The present study was financially supported by Contipro a.s. (Czech Republic).

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The authors declare that they have no conflict of interest.

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Mrazek, J., Kettou, S., Matuska, V. et al. Photo-crosslinked hyaluronic acid coated upconverting nanoparticles. J Nanopart Res 19, 44 (2017). https://doi.org/10.1007/s11051-017-3751-8

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