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Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging

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Abstract

Hyaluronan (HA) represents an interesting polymer for nanoparticle coating due to its biocompatibility and enhanced cell interaction via CD44 receptor. Here, we describe incorporation of oleate-capped β–NaYF4:Yb3+, Er3+ nanoparticles (UCNP-OA) into amphiphilic HA by microemulsion method. Resulting structures have a spherical, micelle-like appearance with a hydrodynamic diameter of 180 nm. UCNP-OA-loaded HA micelles show a good stability in PBS buffer and cell culture media. The intensity of green emission of UCNP-OA-loaded HA micelles in water is about five times higher than that of ligand-free UCNP, indicating that amphiphilic HA effectively protects UCNP luminescence from quenching by water molecules. We found that UCNP-OA-loaded HA micelles in concentrations up to 50 μg mL−1 increase cell viability of normal human dermal fibroblasts (NHDF), while viability of human breast adenocarcinoma cells MDA–MB–231 is reduced at these concentrations. The utility of UCNP-OA-loaded HA micelles as a bio-imaging probe was demonstrated in vitro by successful labelling of NHDF and MDA–MB–231 cells overexpressing the CD44 receptor.

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Acknowledgments

The authors would like to thank Klara Slezingrova and Martin Bobek for synthesizing amphiphilic hyaluronan derivatives, Ondrej Zidek for performing STEM analysis, Pavel Odraska for ICP–OES measurements and Martin Cepa for help with luminescence imaging.

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Authors and Affiliations

  1. Contipro Biotech, Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic

    Martina Pospisilova, Jiri Mrazek, Vit Matuska, Sofiane Kettou, Monika Dusikova, Vit Svozil, Kristina Nesporova, Gloria Huerta-Angeles, Hana Vagnerova & Vladimir Velebny

  2. Department of Medical Biophysics and Medical Informatics, 3rd Medical Faculty, Charles University, Prague, Czech Republic

    Jiri Mrazek

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  1. Martina Pospisilova
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  2. Jiri Mrazek
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Correspondence to Martina Pospisilova.

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Pospisilova, M., Mrazek, J., Matuska, V. et al. Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging. J Nanopart Res 17, 383 (2015). https://doi.org/10.1007/s11051-015-3186-z

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