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Silica nanoparticles mediated neuronal cell death in corpus striatum of rat brain: implication of mitochondrial, endoplasmic reticulum and oxidative stress

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Abstract

Extensive uses of silica nanoparticles (SiNPs) in biomedical and industrial fields have increased the risk of exposure, resulting concerns about their safety. We focussed on some of the safety aspects by studying neurobehavioural impairment, oxidative stress (OS), neurochemical and ultrastructural changes in corpus striatum (CS) of male Wistar rats exposed to 80-nm SiNPs. Moreover, its role in inducing mitochondrial and endoplasmic reticulum (ER) stress-mediated neuronal apoptosis was also investigated. The results demonstrated impairment in neurobehavioural indices, and a significant increase in lipid peroxide levels (LPO), hydrogen peroxide (H2O2), superoxide (O2 -) and protein carbonyl content, whereas there was a significant decrease in the activities of the enzymes, manganese superoxide dismutase (Mn SOD), glutathione peroxidase (GPx), catalase (CAT) and reduced glutathione (GSH) content, suggesting impaired antioxidant defence system. Protein (cytochrome c, Bcl-2, Bax, p53, caspase-3, caspase 12 and CHOP/Gadd153) and mRNA (Bcl-2, Bax, p53 and CHOP/Gadd153, cytochrome c) expression studies of mitochondrial and ER stress-related apoptotic factors suggested that both the cell organelles were involved in OS-mediated apoptosis in treated rat brain CS. Moreover, electron microscopic studies clearly showed mitochondrial and ER dysfunction. In conclusion, the result of the study suggested that subchronic SiNPs’ exposure has the potential to alter the behavioural activity and also to bring about changes in biochemical, neurochemical and ultrastructural profiles in CS region of rat brain. Furthermore, we also report SiNPs-induced apoptosis in CS, through mitochondrial and ER stress-mediated signalling.

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Acknowledgments

We gratefully acknowledge the financial support received from Council of Science & Technology U. P. for carrying out the study.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry, King George’s Medical University, Lucknow, 226003, Uttar Pradesh, India

    Arshiya Parveen, Syed Husain Mustafa Rizvi, Ranjana Singh & Abbas Ali Mahdi

  2. Department of Biochemistry, Era’s Lucknow Medical College & Hospital, Lucknow, India

    Farzana Mahdi

  3. Department of Biotechnology, Institute of Engineering and Technology, Jaipur, India

    Sandeep Tripathi

  4. Fibre Toxicology, CSIR-Indian Institute of Toxicology Research, Lucknow, India

    Iqbal Ahmad

  5. Developmental Toxicology, CSIR-Indian Institute of Toxicology Research, Lucknow, India

    Rajendra K. Shukla & Vinay K. Khanna

  6. Analytical Chemistry Division, CSIR-Indian Institute of Toxicology Research, Lucknow, India

    Devendra K. Patel

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  1. Arshiya Parveen
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  2. Syed Husain Mustafa Rizvi
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  3. Farzana Mahdi
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  5. Iqbal Ahmad
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  10. Abbas Ali Mahdi
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Correspondence to Abbas Ali Mahdi.

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We dedicate this paper in memory of late Padam Shri Prof. Mahdi Hasan, a renowned neuroscientist who was motivator for this study but unfortunately died on 12th January 2013.

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Parveen, A., Rizvi, S.H.M., Mahdi, F. et al. Silica nanoparticles mediated neuronal cell death in corpus striatum of rat brain: implication of mitochondrial, endoplasmic reticulum and oxidative stress. J Nanopart Res 16, 2664 (2014). https://doi.org/10.1007/s11051-014-2664-z

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