Single-walled carbon nanohorns decorated with semiconductor quantum dots to evaluate intracellular transport

  • Kristen A. Zimmermann
  • David L. InglefieldJr.
  • Jianfei Zhang
  • Harry C. Dorn
  • Timothy E. Long
  • Christopher G. Rylander
  • M. Nichole Rylander
Research Paper

Abstract

Single-walled carbon nanohorns (SWNHs) have great potential to enhance thermal and chemotherapeutic drug efficiencies for cancer therapies. Despite their diverse capabilities, minimal research has been conducted so far to study nanoparticle intracellular transport, which is an important step in designing efficient therapies. SWNHs, like many other carbon nanomaterials, do not have inherent fluorescence properties making intracellular transport information difficult to obtain. The goals of this project were to (1) develop a simple reaction scheme to decorate the exohedral surface of SWNHs with fluorescent quantum dots (QDs) and improve conjugate stability, and (2) evaluate SWNH–QD conjugate cellular uptake kinetics and localization in various cancer cell lines of differing origins and morphologies. In this study, SWNHs were conjugated to CdSe/ZnS core/shell QDs using a unique approach to carbodiimide chemistry. Transmission electron microscopy and electron dispersive spectroscopy verified the conjugation of SWNHs and QDs. Cellular uptake kinetics and efficiency were characterized in three malignant cell lines: U-87 MG (glioblastoma), MDA-MB-231 (breast cancer), and AY-27 (bladder transitional cell carcinoma) using flow cytometry. Cellular distribution was verified by confocal microscopy, and cytotoxicity was also evaluated using an alamarBlue assay. Results indicate that cellular uptake kinetics and efficiency are highly dependent on cell type, highlighting the significance of studying nanoparticle transport at the cellular level. Nanoparticle intracellular transport investigations may provide information to optimize treatment parameters (e.g., SWNH concentration, treatment time, etc.) depending on tumor etiology.

Keywords

Single-walled carbon nanohorn (SWNH) Quantum dot (QD) Cellular distribution Uptake kinetics Cancer Nanobiotechnology 

Supplementary material

11051_2013_2078_MOESM1_ESM.pdf (1.9 mb)
Supplementary material 1 (PDF 1911 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kristen A. Zimmermann
    • 1
  • David L. InglefieldJr.
    • 2
  • Jianfei Zhang
    • 3
  • Harry C. Dorn
    • 4
  • Timothy E. Long
    • 3
  • Christopher G. Rylander
    • 1
    • 5
  • M. Nichole Rylander
    • 1
    • 5
  1. 1.School of Biomedical Engineering and SciencesVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  2. 2.Department of ChemistryVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  3. 3.Department of ChemistryVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  4. 4.Department of ChemistryVirginia Tech Carilion Research InstituteRoanokeUSA
  5. 5.Department of Mechanical EngineeringVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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