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Dual functions of polyvinyl alcohol (PVA): fabricating particles and electrospinning nanofibers applied in controlled drug release

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Abstract

The fabrication of submicron size microsphere from 8-Phe-4 poly(ester amide) (PEA) using polyvinyl alcohol (PVA) as the emulsion was reported. The biodegradable microspheres were prepared by an oil-in-water emulsion/solvent evaporation technique, and PVA was used as the emulsion. Furthermore, the emulsion PVA was electrospun into nanofibrous mats, and 8-Phe-4 PEA microspheres were entrapped in the resultant mats. The dual functions of PVA to fabricate ideal nanofibrous mats which can entrap microspheres in them and to obtain 8-Phe-4 microspheres as emulsion in their potential application were demonstrated. The anti-cancer drug doxorubicin (DOX) was encapsulated in the 8-Phe-4 amino acid-based PEA microspheres and the entrapment efficiency is almost 100 %. At the same time, the DOX can be controlled released in PBS solution and in α-chymotrypsin solution. The cytotoxicity of PVA, PVA mats-entrapped 8-Phe-4 microspheres and PVA mats-entrapped DOX-loaded 8-Phe-4 microspheres, was investigated. Hela cells were used to test the cytotoxicity of the DOX that released from the PVA mats-entrapped DOX-loaded 8-Phe-4 microspheres for 2 days, and the cell viability is below 30 % when the 8-Phe-4 microspheres concentration is 1 mg/mL. It demonstrated that the PVA mats-entrapped DOX-loaded 8-Phe-4 microspheres have a potential biomedical application.

Graphical Abstract

The table of contents: DOX-loaded microspheres can be encapsulated in the PVA fibers by electrospinning and the DOX can be controlled released from the PVA fibers-entrapped microspheres. MTT assay indicated that the more than 70 % Hela cells were killed by the DOX released from DOX-loaded microspheres encapsulated in the PVA after 48 h.

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Acknowledgments

This work was partially supported by the grants from the National Natural Science Foundation of China (50973014, 11172064 and 81101241), the Foundation for the Author of National Excellent Doctoral Dissertation of P. R. China (200961), and Shanghai Rising-Star Program in China (10QA1400100) and Fok Ying Tong Education Foundation (121071) to Prof. Xiao-Hong Qin and also Supported by Program for New Century Excellent Talents in University (NCET-10-0322) and the Fundamental Research Funds for the Central Universities as well as “Shu Guang” (11SG33) project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation to her.

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Authors and Affiliations

  1. College of Textiles, Donghua University, No. 2999 North Renmin Rd. Songjiang, Shanghai, 201620, China

    Xiao-Hong Qin

  2. Department of Fiber Science and Apparel Design, Cornell University, Ithaca, NY, 14853-4401, USA

    Xiao-Hong Qin, De-Qun Wu & Chih-Chang Chu

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  1. Xiao-Hong Qin
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  2. De-Qun Wu
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  3. Chih-Chang Chu
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Correspondence to Xiao-Hong Qin or Chih-Chang Chu.

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Qin, XH., Wu, DQ. & Chu, CC. Dual functions of polyvinyl alcohol (PVA): fabricating particles and electrospinning nanofibers applied in controlled drug release. J Nanopart Res 15, 1395 (2013). https://doi.org/10.1007/s11051-012-1395-2

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