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Using fluorescence measurement of zinc ions liberated from ZnS nanoparticle labels in bioassay for Escherichia coli O157:H7

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Abstract

In this study, an alternative approach using ZnS nanoparticle biolabels as fluorescence signal transducers is reported for the immunoassay of E. coli O157:H7 in tap water samples. Instead of measuring the fluorescence of ZnS nanoparticles in the assay, the fluorescence signal is generated through the binding of zinc ions released from nanoparticle labels with zinc-ion sensitive fluorescence indicator Fluozin-3. In the assay, ZnS nanoparticles around 50 nm in diameter were synthesized, bioconjugated, and applied for the detection of E. coli O157:H7. The assay shows a detection range over two orders of magnitude and a detection limit around 1000 colony-forming units (cfu) of E. coli O157:H7.

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Acknowledgments

The authors would like to thank Dr. Mojtaba Ahmadian-Tehrani (Department of Chemical and Materials Engineering at the University of Nevada Reno) for his technical support in SEM imaging. C.C. expresses gratitude to the University of Nevada Reno for scholarship.

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Authors and Affiliations

  1. Department of Electrical and Biomedical Engineering, University of Nevada Reno, Reno, NV, 89557-0260, USA

    Chad L. Cowles & Xiaoshan Zhu

  2. Department of Biology, University of Nevada Reno, Reno, NV, 89557-0314, USA

    Chi-Yun Pai

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  1. Chad L. Cowles
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  2. Xiaoshan Zhu
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  3. Chi-Yun Pai
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Correspondence to Xiaoshan Zhu.

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Cowles, C.L., Zhu, X. & Pai, CY. Using fluorescence measurement of zinc ions liberated from ZnS nanoparticle labels in bioassay for Escherichia coli O157:H7. J Nanopart Res 13, 5407–5413 (2011). https://doi.org/10.1007/s11051-011-0528-3

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  • DOI: https://doi.org/10.1007/s11051-011-0528-3

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