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GoldMag nanoparticles with core/shell structure: characterization and application in MR molecular imaging

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Abstract

GoldMag is a kind of bi-functional nanoparticle, composed of a gold nanoshell and an iron oxide core. GoldMag combines the antibody immobilization property of gold nanoshell with the superparamagnetic feature of the iron oxide core. Rabbit anti-mouse IgG was immobilized on the surface of GoldMag to synthesize GoldMag-IgG in a single-step process. Transmission electron microscopy, UV/Vis spectrophotometry, zeta potential analysis, dynamic light scattering, enzyme-linked immunosorbent assay, and magnetic resonance imaging (MRI) were employed to characterize the nanostructures and the spectroscopic and magnetic properties of GoldMag and GoldMag-IgG. The antibody encapsulation efficiency of GoldMag was measured as 58.7%, and the antibody loading capacity was 88 μg IgG per milligram of GoldMag. The immunoactivity of GoldMag-IgG was estimated to be 43.3% of that of the original IgG. The cytotoxicity of GoldMag was assessed by MTT assay, which showed that it has only little influence on human dermal lymphatic endothelial cells. MR imaging of different concentrations of ultrasmall superparamagnetic iron oxide, GoldMag, and GoldMag-IgG showed that 3 μg/mL of nanoparticles could significantly affect the MRI signal intensity of GRE T2*WI. The results demonstrate that GoldMag nanoparticles can be effectively conjugated with biomacromolecules and possess great potential for MR molecular imaging.

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Acknowledgments

The authors would like to express thanks to Prof. Wei Sun for reviewing the manuscript. The study was financed by the National Natural Science Foundation of China (Grant Nos. 30770609, 81071197).

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Authors and Affiliations

  1. Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

    Song Zhang, Liguang Zou, Dong Zhang, Xin Pang & Hua Yang

  2. Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

    Ying Xu

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  1. Song Zhang
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  2. Liguang Zou
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  3. Dong Zhang
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  4. Xin Pang
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  5. Hua Yang
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Correspondence to Liguang Zou.

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Zhang, S., Zou, L., Zhang, D. et al. GoldMag nanoparticles with core/shell structure: characterization and application in MR molecular imaging. J Nanopart Res 13, 3867–3876 (2011). https://doi.org/10.1007/s11051-011-0339-6

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  • DOI: https://doi.org/10.1007/s11051-011-0339-6

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