Skip to main content
SpringerLink
Log in

Production of anti-fullerene C60 polyclonal antibodies and study of their interaction with a conjugated form of fullerene

  • Research Paper
  • Published:
Journal of Nanoparticle Research Aims and scope Submit manuscript

Abstract

The aim of this study was to produce anti-fullerene C60 antibodies for the development of detection systems for fullerene C60 derivatives. To produce anti-fullerene C60 antibodies, conjugates of the fullerene C60 carboxylic derivative with thyroglobulin, soybean trypsin inhibitor, and bovine serum albumin were synthesized by carbodiimide activation and characterized. Immunization of rabbits by the conjugates led to the production of polyclonal anti-fullerene antibodies. The specificity of the immune response to fullerene was investigated. Indirect competitive immunoenzyme assay was developed for the determination of conjugated fullerene with detection limits of 0.04 ng/mL (calculated for coupled C60) and 0.4 ng/mL (accordingly to total fullerene–protein concentration).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

BSA:

Bovine serum albumin

2,4-D:

2,4-dichlorophenoxyacetic acid

ELISA:

Enzyme-linked immunosorbent assay

PBS:

Phosphate buffered saline (50 mM potassium phosphate buffer, pH 7.4, with 0.1 M NaCl)

STI:

Soybean trypsin inhibitor

PTG:

Porcine thyroglobulin

References

  • Andreev SM, Babakhin AA, Petrukhina AO, Romanova VS, Parnes ZN, Petrov RV (2000) Immunogenic and allergenic properties of fulleren conjugates with aminoacids and proteins. Dokl Biochem 370(1–6):261–264

    CAS  Google Scholar 

  • Benyamini H, Shulman-Peleg A, Wolfson HJ, Belgorodsky B, Fadeev L, Gozin M (2006) Interaction of C60-fullerene and carboxyfullerene with proteins: docking and binding site alignment. Bioconjug Chem 17(2):378–386

    Article  CAS  Google Scholar 

  • Bouchard D, Ma X (2008) Extraction and high-performance liquid chromatographic analysis of C60, C70, and [6,6]-phenyl C61-butyric acid methyl ester in synthetic and natural waters. J Chromatogr A 1203(2):153–159

    Article  CAS  Google Scholar 

  • Braden BC, Goldbaum FA, Chen B-X, Kirschner AN, Wilson SR, Erlanger BF (2000) X-ray crystal structure of an anti-buckminsterfullerene antibody Fab fragment: biomolecular recognition of C60. Proc Natl Acad Sci USA 97(22):12193–12197

    Article  CAS  Google Scholar 

  • Chen B-X, Wilson SR, Das M, Coughlin DJ, Erlanger BF (1998) Antigenicity of fullerenes: antibodies specific for fullerenes and their characteristics. Proc Natl Acad Sci USA 95(18):10809–10813

    Article  CAS  Google Scholar 

  • Deguchi S, Yamazaki T, Mukai SA, Usami R, Horikoshi K (2007) Stabilization of C60 nanoparticles by protein adsorption and its implications for toxicity studies. Chem Res Toxicol 20(6):854–858

    Article  CAS  Google Scholar 

  • Deye JR, Shiveley AN, Oehrle SA, Walters KA (2008) Separation of substituted fullerenes using non-aqueous reversed-phase liquid chromatography-mass spectrometry. J Chromatogr A 1181(1–2):159–161

    Article  CAS  Google Scholar 

  • Dzantiev BB, Zherdev AV, Romanenko OG, Sapegova LA (1996) Development and comparative study of different immunoenzyme techniques for pesticide detection. Int J Environ Anal Chem 65(1–4):95–111

    CAS  Google Scholar 

  • Erlanger BF, Chen B-X, Zhu M, Brus L (2001) Binding of an anti-fullerene IgG monoclonal antibody to single wall carbon nanotubes. Nano Lett 1(9):465–467

    Article  CAS  Google Scholar 

  • Habeeb AF (1966) Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. Anal Biochem 14(3):328–336

    Article  CAS  Google Scholar 

  • Hendrickson OD, Pen’kova (Fedyunina) NS, Zherdev AV, Dzantiev BB (2009) The production of antibodies specific for fullerenes C60. In: Abstracts of the 2nd international forum on nanotechnologies, Moscow, Russia, 6–8 Oct, p 227

  • Hermanson GT (2008) Bioconjugate techniques. Elsevier, Academic Press, Amsterdam, London

    Google Scholar 

  • Isaacson CW, Kleber M, Field JA (2009) Quantitative analysis of fullerene nanomaterials in environmental systems: a critical review. Environ Sci Technol 43(17):6463–6474

    Article  CAS  Google Scholar 

  • Kahru A, Dubourguier HC (2010) From ecotoxicology to nanoecotoxicology. Toxicology 269:105–119

    Article  CAS  Google Scholar 

  • Kotelnikova RA, Bogdanov GN, Frog EC, Kotelnikov AI, Shtolko VN, Romanova VS, Andreev SM, Kushch AA, Fedorova NE, Medzhidova AA, Miller GG (2003) Nanobionics of pharmacologically active derivatives of fullerene C60. J Nanopart Res 5(5–6):561–566

    Google Scholar 

  • Moussa F, Pressac M, Genin E, Roux S, Trivin F, Rassat A, Céolin R, Szwarc H (1997) Quantitative analysis of C60 fullerene in blood and tissues by high-performance liquid chromatography with photodiode-array and mass spectrometric detection. J Chromatogr B 696(1):153–159

    Article  CAS  Google Scholar 

  • Partha R, Conyers JL (2009) Biomedical applications of functionalized fullerene-based nanomaterials. Int J Nanomed 4:261–275

    Article  CAS  Google Scholar 

  • Ruoff RS, Tse DS, Malhorta R, Lorents DC (1993) Solubility of C60 in a variety of solvents. J Phys Chem 97(13):3379–3383

    Article  CAS  Google Scholar 

  • Sittampalam GS, Smith WC, Miyakawa TW, Smith DR, McMorris C (1996) Application of experimental design techniques to optimize a competitive ELISA. J Immunol Methods 190(2):151–161

    Article  CAS  Google Scholar 

  • Tiede K, Hassellöv M, Breitbarth E, Chaudhry Q, Boxall AB (2009) Considerations for environmental fate and ecotoxicity testing to support environmental risk assessments for engineered nanoparticles. J Chromatogr A 1216(3):503–509

    Article  CAS  Google Scholar 

  • Xia XR, Monteiro-Riviere NA, Riviere JE (2006) Trace analysis of fullerenes in biological samples by simplified liquid–liquid extraction and high-performance liquid chromatography. J Chromatogr A 1129(2):216–222

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the Federal Target Program “Development of Nanoindustry Infrastructure in the Russian Federation for 2008–2011.”

Author information

Authors and Affiliations

  1. Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia

    O. D. Hendrickson, N. S. Fedyunina, A. V. Zherdev & B. B. Dzantiev

  2. Moscow State University, Leninskie gory, 119991, Moscow, Russia

    A. A. Martianov

Authors
  1. O. D. Hendrickson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. S. Fedyunina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Martianov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. V. Zherdev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. B. Dzantiev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. D. Hendrickson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hendrickson, O.D., Fedyunina, N.S., Martianov, A.A. et al. Production of anti-fullerene C60 polyclonal antibodies and study of their interaction with a conjugated form of fullerene. J Nanopart Res 13, 3713–3719 (2011). https://doi.org/10.1007/s11051-011-0292-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11051-011-0292-4

Keywords

Search

Navigation