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Nanosized cancer cell-targeted polymeric immunomicelles loaded with superparamagnetic iron oxide nanoparticles

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Abstract

Stable 30–50 nm polymeric polyethylene glycol–phosphatidylethanolamine (PEG–PE)-based micelles entrapping superparamagnetic iron oxide nanoparticles (SPION) have been prepared. At similar concentrations of SPION, the SPION-micelles had significantly better magnetic resonance imaging (MRI) T2 relaxation signal compared to ‘plain’ SPION. Freeze-fracture electron microscopy confirmed SPION entrapment in the lipid core of the PEG–PE micelles. To enhance the targeting capability of these micelles, their surface was modified with the cancer cell-specific anti-nucleosome monoclonal antibody 2C5 (mAb 2C5). Such mAb 2C5-SPION immunomicelles demonstrated specific binding with cancer cells in vitro and were able to bring more SPION to the cancer cells thus demonstrating the potential to be used as targeted MRI contrast agents for tumor imaging.

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Acknowledgment

This work was supported by the NIH grants RO1 EB01961 and RO1 EN002995 to Vladimir P. Torchilin and by Northeastern University and NSF grant DGE-0504331 to Srinivas Sridhar.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Mugar Building, Room 312, 360 Huntington Avenue, Boston, MA, 02115, USA

    Rishikesh M. Sawant, Rupa R. Sawant & Vladimir P. Torchilin

  2. Electronic Materials Research Institute, Northeastern University, Boston, MA, 02115, USA

    Evin Gultepe, Dattatri Nagesha & Srinivas Sridhar

  3. Nano Analytical Laboratory, 3951 Sacramento Street, San Francisco, CA, 94118, USA

    Brigitte Papahadjopoulos-Sternberg

Authors
  1. Rishikesh M. Sawant
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  2. Rupa R. Sawant
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  3. Evin Gultepe
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  4. Dattatri Nagesha
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  5. Brigitte Papahadjopoulos-Sternberg
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  6. Srinivas Sridhar
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  7. Vladimir P. Torchilin
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Correspondence to Vladimir P. Torchilin.

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Sawant, R.M., Sawant, R.R., Gultepe, E. et al. Nanosized cancer cell-targeted polymeric immunomicelles loaded with superparamagnetic iron oxide nanoparticles. J Nanopart Res 11, 1777–1785 (2009). https://doi.org/10.1007/s11051-009-9611-4

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  • DOI: https://doi.org/10.1007/s11051-009-9611-4

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