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Antifungal Activities of Different Extracts of Marine Macroalgae Against Dermatophytes and Candida Species

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Abstract

Algae are bioactive natural resources, and due to the medical importance of superficial mycoses, we focused the action of macroalgae extracts against dermatophytes and Candida species. Seaweed obtained from the Riacho Doce beach, Alagoas (Brazil), were screened for the antifungal activity, through crude extracts using dichloromethane, chloroform, methanol, ethanol, water and chloroform and hexane fractions of green, brown and red algae in assays with standard strains of the dermatophytes Trichophyton rubrum, T. tonsurans, T. mentagrophytes, Microsporum canis, M. gypseum and yeasts Candida albicans, C. krusei, C. guilliermondi and C. parapsilosis. The M44-A and M27-A2/M38A manuals by CLSI were followed, and the minimum inhibitory concentration (MIC) ranged from 0.03 to 16.00 μg ml−1, and an inhibition halo of 10.00–25.00 mm was observed for dermatophytes, while for yeast, it was from 8.00 to 16.00 μg ml−1 and 10.00–15.00 mm. M. canis showed MIC of 0.03 μg ml−1 and the largest inhibition halo in T. rubrum (25.00 mm) through the use of the methanol extract. For C. albicans, dichloromethane, methanol and ethanol extracts formed the largest inhibition halo. The ethanol extract was shown to be the best inhibiting fungi growth, and chloroform and hexane fractions of H. musciformis inhibited the growth of all dermatophytes and C. albicans, yielding the conclusion that apolar extracts obtained from algae presented the best activity against important pathogenic fungi.

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Acknowledgments

We are grateful to Ana Maria Queijeiro Lopes (IQB/UFAL) and Eurípedes Alves Silva Filho (ICBS/UFAL) for suggestions, Júlio César Voltolini for support in statistical analysis and to Abilio Borghi for the English review on the manuscript. This work was supported by grants from the CAPES.

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There is no conflict of interest in this paper, and all authors agree to publication.

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Authors and Affiliations

  1. Instituto de Química e Biotecnologia, Laboratório de Pesquisa em Recursos Naturais/IQB and Laboratório de Ficologia/ICBS , Universidade Federal de Alagoas, Maceió, Alagoas, 57072-970, Brazil

    Elica Amara Cecilia Guedes

  2. Faculdade de Ciências Biológicas e da Saúde, Laboratório de Micologia, Centro Universitário Cesmac, Maceió, Alagoas, 57051-160, Brazil

    Maria Anilda dos Santos Araújo, Aryanna Kelly Pinheiro Souza & Larissa Isabela Oliveira de Souza

  3. Instituto de Química e Biotecnologia, Laboratório de Pesquisa em Recursos Naturais/IQB, Universidade Federal de Alagoas, Maceió, Alagoas, 57072-970, Brazil

    Lurdiana Dayse de Barros & Antônio Euzébio Goulart Sant’Ana

  4. Instituto de Ciências Biológicas e da Saúde, Laboratório de Microbiologia Geral e Clínica, Universidade Federal de Alagoas, Maceió, Alagoas, 57010-020, Brazil

    Fernanda Cristina de Albuquerque Maranhão

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  1. Elica Amara Cecilia Guedes
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Correspondence to Elica Amara Cecilia Guedes.

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Guedes, E.A.C., dos Santos Araújo, M.A., Souza, A.K.P. et al. Antifungal Activities of Different Extracts of Marine Macroalgae Against Dermatophytes and Candida Species. Mycopathologia 174, 223–232 (2012). https://doi.org/10.1007/s11046-012-9541-z

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  • DOI: https://doi.org/10.1007/s11046-012-9541-z

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