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Enzymatic Activity, Sensitivity to Antifungal Drugs and Baccharis dracunculifolia Essential Oil by Candida Strains Isolated from the Oral Cavities of Breastfeeding Infants and in Their Mothers’ Mouths and Nipples

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Abstract

Candida strains can cause oral candidosis, as well as nipples candidosis and lead to premature weaning or yeast transmission. The aim of this study was to evaluate 51 Candida isolates obtained from the oral cavities of infants during breastfeeding and mothers’ oral cavities and nipples, their enzymatic activity and their sensitivity to amphotericin B, fluconazole and Baccharis dracunculifolia essential oil. Among the studied strains, 96.1% produced phospholipase and 78.4% produced proteinase. The antifungal resistance was only observed among isolates of C. albicans, for which three strains showed a resistant activity to fluconazole and one showed a resistant activity to amphotericin B. All strains were sensitive to B. dracunculifolia essential oil with MIC between 0.2 and 6.25 mg/ml. It was concluded that most of the strains showed significant enzymatic activity and were sensitive to amphotericin B and fluconazole. B. dracunculifolia essential oil inhibited the growth of all strains, including the ones resistant to commercial antifungal agents.

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Acknowledgments

The authors Cristiane A. Pereira and Anna Carolina B. P. Costa are grateful to FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) by the scholarships provided (Processes 2010/00879-4 and 2009/12005-1). Authors are thankful to PharmaNéctar® (Belo Horizonte—Brazil) for the donation of Baccharis dracunculifolia leaves.

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Authors and Affiliations

  1. Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, São José dos Campos Dental School, São Paulo State University (UNESP), Avenida Engenheiro Francisco José Longo, 777, São Dimas, São José dos Campos, CEP 12245-000, São Paulo, Brazil

    Cristiane Aparecida Pereira, Anna Carolina Borges Pereira da Costa, Ana Karina Silva Machado, Juliana Campos Junqueira & Antonio Olavo Cardoso Jorge

  2. Biosciences Institute, Taubaté University (UNITAU), Taubaté, São Paulo, Brazil

    Maria Stella Amorin Costa Zöllner

  3. Institute of Research and Development, Vale do Paraíba University (UNIVAP), São José dos Campos, São Paulo, Brazil

    Miltom Beltrame Júnior

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  1. Cristiane Aparecida Pereira
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Correspondence to Antonio Olavo Cardoso Jorge.

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Pereira, C.A., da Costa, A.C.B.P., Machado, A.K.S. et al. Enzymatic Activity, Sensitivity to Antifungal Drugs and Baccharis dracunculifolia Essential Oil by Candida Strains Isolated from the Oral Cavities of Breastfeeding Infants and in Their Mothers’ Mouths and Nipples. Mycopathologia 171, 103–109 (2011). https://doi.org/10.1007/s11046-010-9353-y

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