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In Vitro Evaluation of Putative Virulence Attributes of Oral Isolates of Candida spp. Obtained from Elderly Healthy Individuals

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Abstract

Identification of Candida isolates obtained from oral cavity of elderly healthy individuals revealed the predominance of non-albicans Candida species (88.9%) compared to Candida albicans (11%). CHROMagar Candida differential medium and PCR revealed the presence of Candida tropicalis (33.3%), Candida glabrata (27.8%), and Candida krusei (16.7%). We investigated the presence of virulence attributes in a total of 18 isolates, including acid protease and phospholipase production, hemolytic activity, and biofilm production. Extracellular protease was found in five isolates (27.8%) whereas extracellular phospholipase was found in three isolates (17%). All isolates showed hemolytic activity. About 56% of the isolates were weakly positive for biofilm formation (score +) whereas a minority (5.6%) of them showed strong biofilm formation (score 4+). Susceptibility in vitro of the isolates to fluconazole was carried out by microdilution method. Fluconazole showed a strong inhibition against most buccal isolates. The resistant isolates were 2 C. tropicalis, 2 C. glabrata, and 1 C. krusei.

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Acknowledgments

This work was supported in part by Fundação Araucária—Paraná—Brazil. F.C.B. was fellowship-holder of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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  1. Universidade Tecnológica Federal do Paraná, Rua Alagoas, 2001, Cep 86020-430, Londrina, PR, Brazil

    Luciana Furlaneto-Maia, Ana Flavia Specian, Fernando Cesar Bizerra, Marcelo Tempesta de Oliveira & Márcia Cristina Furlaneto

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  1. Luciana Furlaneto-Maia
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  2. Ana Flavia Specian
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  3. Fernando Cesar Bizerra
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  4. Marcelo Tempesta de Oliveira
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Correspondence to Luciana Furlaneto-Maia.

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Furlaneto-Maia, L., Specian, A.F., Bizerra, F.C. et al. In Vitro Evaluation of Putative Virulence Attributes of Oral Isolates of Candida spp. Obtained from Elderly Healthy Individuals. Mycopathologia 166, 209–217 (2008). https://doi.org/10.1007/s11046-008-9139-7

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