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Gene expression profiling of vitamin D metabolism enzymes in leukemia and lymphoma patients: molecular aspect interplay of VDR, CYP2R1, and CYP24A1

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Abstract

Background

Vitamin D deficiency is prevalent among the Indonesian population, particularly in individuals diagnosed with leukemia-lymphoma. The regulation of vitamin D metabolism is influenced by the expression of several enzymes, such as CYP2R1, CYP24A1, and the vitamin D receptor (VDR). This study aimed to scrutinize the gene expression profiles in both mRNA and protein levels of VDR, CYP2R1, and CYP24A1 in leukemia and lymphoma patients.

Method

The research was a cross-sectional study conducted at Cipto Mangunkusumo Hospital (RSCM) in Jakarta, Indonesia. The study included a total of 45 patients aged over 18 years old who have received a diagnosis of lymphoma or leukemia. Vitamin D status was measured by examining serum 25 (OH) D levels. The analysis of VDR, CYP2R1, and CYP24A1 mRNA expression utilized the qRT-PCR method, while protein levels were measured through the ELISA method.

Conclusion

The study revealed a noteworthy difference in VDR protein levels between men and women. The highest mean CYP24A1 protein levels were observed in the age group > 60 years. This study found a significant, moderately positive correlation between VDR protein levels and CYP24A1 protein levels in the male and vitamin D sufficiency groups. In addition, a significant positive correlation was found between VDR mRNA levels and CYP2R1 mRNA levels, VDR mRNA levels and CYP2R1 mRNA levels, and CYP2R1 mRNA levels and CYP24A1 mRNA levels. However, the expression of these genes does not correlate with the protein levels of its mRNA translation products in blood circulation.

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Data availability

The datasets used and/or analyzed during the current study are available in our institutionalized database and the Copy Right of this study has been deposited in the repository of Yarsi University, or please contact the corresponding author upon reasonable request.

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Acknowledgements

We would like to thank the following hospital management: Yarsi Hospital, and Cipto Mangunkusumo National General Hospital.

Funding

This research was funded by the Rumah Program Organization of Health National Research and Innovation Agency (BRIN), Grant number: B-231/III.9/PR.03.08/1/2023.

Author information

Authors and Affiliations

  1. Department of Clinical Pathology, Faculty of Medicine, Yarsi University, Yarsi Hospital, Jl. Letjen Suprapto Kav 13, Cempaka Putih, Jakarta, 10510, Indonesia

    Anggraini Iriani

  2. Department of Hematology Oncology, Faculty of Medicine, Cipto Manguskusumo Hospital, Jakarta, Indonesia

    Andhika Rachman

  3. Faculty of Medicine, Andalas University, Padang, Indonesia

    Marsya Kaila Fatina

  4. Faculty of Medicine, Yarsi University, Jakarta, Indonesia

    Rizka Kurnia Gemilang

  5. Genomics Laboratory, Bunda Hospital, Jakarta, Indonesia

    Andi Trisnandi

  6. Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Cibinong, Indonesia

    Media Fitri Isma Nugraha

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  1. Anggraini Iriani
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Contributions

AI, AR, and MFIN performed study concept and development of methodology; AI, MFIN, RKG, and AT performed validation; AR, AI, and RKG provided resource and investigation; AI, RKG, AT, and MKF provided data curation and performed formal analysis; AR, AI, RKG, and MKF performed writing original draft preparations; AI, MKF, and MFIN performed writing, review and editing of the paper; AI and MFIN provided supervision; MFIN performed project administration and founding acquisition. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Anggraini Iriani.

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Competing interest

The authors declare no conflict of interest regarding this study.

Ethical approval

This study has received ethical approval from the Ethics Committee of the Faculty of Medicine Yarsi University No: 030/KEP-UY/EA.20/II/2023. This study was conducted following the declaration of Helsinki. Moreover, informed consent forms were duly signed by all participants.

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Iriani, A., Rachman, A., Fatina, M.K. et al. Gene expression profiling of vitamin D metabolism enzymes in leukemia and lymphoma patients: molecular aspect interplay of VDR, CYP2R1, and CYP24A1. Mol Biol Rep 51, 526 (2024). https://doi.org/10.1007/s11033-024-09432-6

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