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Functional analysis of a rice 12-oxo-phytodienoic acid reductase gene (OsOPR1) involved in Cd stress tolerance

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Abstract

Background

The accumulation of cadmium (Cd) in plants may compromise the growth and development of plants, thereby endangering human health through the food chain. Understanding how plants respond to Cd is important for breeding low-Cd rice cultivars.

Methods

In this study, the functions of 12-oxo-phytodienoic acid reductase 1 (OsOPR1) were predicted through bioinformatics analysis. The expression levels of OsOPR1 under Cd stress were analyzed by using qRT-PCR. Then, the role that OsOPR1 gene plays in Cd tolerance was studied in Cd-sensitive yeast strain (ycf1), and the Cd concentration of transgenic yeast was analyzed using inductively coupled plasma mass spectrometry (ICP-MS).

Results

Bioinformatics analysis revealed that OsOPR1 was a protein with an Old yellow enzyme-like FMN (OYE_like_FMN) domain, and the cis-acting elements which regulate hormone synthesis or responding abiotic stress were abundant in the promoter region, which suggested that OsOPR1 may exhibit multifaceted biological functions. The expression pattern analysis showed that the expression levels of OsOPR1 were induced by Cd stress both in roots and roots of rice plants. However, the induced expression of OsOPR1 by Cd was more significant in the roots compared to that in roots. In addition, the overexpression of OsOPR1 improved the Cd tolerance of yeast cells by affecting the expression of antioxidant enzyme related genes and reducing Cd content in yeast cells.

Conclusion

Overall, these results suggested that OsOPR1 is a Cd-responsive gene and may has a potential for breeding low-Cd or Cd-tolerant rice cultivars and for phytoremediation of Cd-contaminated in farmland.

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Data availability

The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

ABA:

Abscisic acid

ABRE:

Abscisic acid response element

AOC:

Allene oxide cyclase

A.thaliana :

Arabidopsis thaliana

ARE:

Antioxidant response element

Cd:

Cadmium

E. coli :

Escherichia coli

FMN:

Flavin mononucleotide

GA:

Gibberellic acid

GMQE:

Global model quality estimate

H2O2 :

Hydrogen peroxide

ICP- MS:

Inductively coupled plasma mass spectrometry

JA:

Jasmonic acid

MBS:

Drought stress response element

MS:

Murashige and Skoog

NJ:

Neighbor- Joining

OPR:

The 12- oxo- phytodienoic acid reductas

OYE_like_FMN:

Old yellow enzyme- like FMN

OYEs:

Old yellow enzymes

OPR3:

OPC- 8:0 by 12- oxophytodienoate reductase 3

O3 :

Ozone

PPI:

Protein- protein interaction

PCR:

Polymerase Chain Reaction

qRT- PCR:

Quantitative real time polymerase chain reaction

SD- Ura:

Synthetic dropout - Uracil medium

UBQ:

Ubiquitin

UV:

Ultraviolet

4CLL4:

4- coumarate- CoA ligase- like4

4CLL8:

4- coumarate- CoA ligase- like 8

4CLL:

4- coumarate: CoA ligase- like

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Funding

This work was supported by the National Natural Science Foundation of China (31870383) and Sichuan Provincial Financial “1 + 3” Project (2021ZYGG-002).

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Authors and Affiliations

  1. College of Ecology and Environment, Chengdu University of Technology, Sichuan, 610059, China

    Longying Wu, Ruolin Wang, Mingyu Li, Zhiye Du, Yufan Jin, Yang Shi, Wenjun Jiang, Yuan Jiao & Jin Huang

  2. College of Agronomy, Sichuan Agricultural University, Sichuan, 611130, China

    Ji Chen

  3. Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Sichuan, 610066, China

    Binhua Hu

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  1. Longying Wu
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  2. Ruolin Wang
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  11. Jin Huang
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Contributions

YS: Conceptualization. RW, LW: Methodology. RW, ML: Formal analysis and investigation. LW, ZD: Writing—original draft preparation. YJ, JH, WJ: Writing—review and editing. JC, JH, BH: Funding acquisition. MZ, JY: Supervision. All authors reviewed the manuscript.

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Correspondence to Ji Chen or Jin Huang.

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Wu, L., Wang, R., Li, M. et al. Functional analysis of a rice 12-oxo-phytodienoic acid reductase gene (OsOPR1) involved in Cd stress tolerance. Mol Biol Rep 51, 198 (2024). https://doi.org/10.1007/s11033-023-09159-w

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