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Ribosomal DNA copy number alteration in blood sample from gastric cancer patients

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Abstract

Background

Ribosomal DNA (rDNA) is the most abundant and important housekeeping gene in the cell. It usually acted as DNA damage sensor in DNA damage reaction. Gastric cancer (GC) as a tumor with high morbidity and mortality, it is hard to diagnosis in an early stage.

Methods

In this study, we collected and test the copy number of rDNA in blood sample of 42 GC patients and 56 healthy controls (HC) to explore the relationship between rDNA and GC. Besides, we make a correlation between the copy number of rDNA and ten biomarkers (CYFR21-1, CA15-3, CA72-4, NSE, CEA, CA125, ProGRP, AFP, SCC, CA19-9).

Results

The copy number of 18 S, 5.8 S, 28 S rDNA in GC is less than HC and 5 S is more than HC in their blood sample. And the expression of H-cox-1 and ND1 in GC is higher than HC in blood sample. it shows the expression of CA15-3 is related to ND1 and H-cox-1.

Conclusion

We found for the first time the changes of rDNA and mtDNA expression in the blood of patients with gastric cancer. All these finding suggests rDNA may have potential in diagnosing GC.

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Data availability

The data supporting the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We thank all participants in Zhejiang Provincial People’s Hospital for their help with this research.

Funding

This study was supported by the Zhejiang Provincial Program for Xinmiao Talents (2022R424B009).

Author information

Author notes

  1. Changchang Chen, Lingfang Feng and Junfei Chen equally contributed to this work.

Authors and Affiliations

  1. School of Basic Medicine and Forensic Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, China

    Changchang Chen & Lijun Lin

  2. School of Public Health, Hangzhou Medical College, Hangzhou, Zhejiang, China

    Lingfang Feng & Junfei Chen

  3. Department of Medical Administration, Affiliated People’s Hospital, Zhejiang Provincial People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, China

    Jian Shen

Authors
  1. Changchang Chen
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  2. Lingfang Feng
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  4. Jian Shen
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  5. Lijun Lin
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Contributions

LJL designed and supervised this study; CCC and LFF drafted the manuscript; JFC provided technical and material support and data analysis; CCC and JS provided the samples and clinical data.

Corresponding author

Correspondence to Lijun Lin.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the national research committee, and this study was approved by the Ethics Committee of Zhejiang Provincial People’s Hospital (No. 2022QT010). All samples were anonymous.

Informed consent

All participants involved in this study, including patients, were fully informed about the study and voluntarily provided written informed consent before participating.

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Chen, C., Feng, L., Chen, J. et al. Ribosomal DNA copy number alteration in blood sample from gastric cancer patients. Mol Biol Rep 50, 7155–7160 (2023). https://doi.org/10.1007/s11033-023-08630-y

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  • DOI: https://doi.org/10.1007/s11033-023-08630-y

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