Abstract
Background:
Breast cancer susceptibility is greatly influenced by single nucleotide polymorphisms (SNPs) both in penetrance and non-penetrance genes. The Estrogen Receptor Alfa (ESR1- rs2234693 and rs2046210) have been reported as risk factor of breast cancer in different ethnic groups with inconsistent results. In this study the association of ESR1 (rs2234693 and rs2046210) with breast cancer risk was investigated in patients of Khyber Pakhtunkhwa.
Methods:
A total of 312 females including 162 breast cancer patients and 150 healthy controls were enrolled in this study. The polymorphism was confirmed using T-ARMS-PCR.
Results:
Our results revealed that ESR1-rs2234693 risk allele (C) (P = 0.21, OR = 1.27, CI = 0.87 to 1.87) and containing genotypes CC (P = 0.68, OR = 1.24, CI = 0.42 to 3.68) and TC (P = 0.23, OR = 1.32, CI = 0.83 to 2.13) were not associated with the risk of breast cancer. In case of rs2046210, the risk allele A (P < 0.0001, OR = 2.42, CI = 1.74 to 3.38) and corresponding genotypes GA (P = 0.0001, OR = 2.55, CI = 1.62 to 4.03) and AA (P = 0.02, OR = 2.20, CI = 1.12 to 4.34) were significantly associated with higher risk of breast cancer. Moreover, ESR1-rs2234693 was significantly (P < 0.05) associated with family history, stages, PR status, ER status and luminal B. The ESR1-rs2046210 showed significant (P ≤ 0.05) association with menstrual status, tumor grade and TNBC. Both the SNPs showed non-significant (P > 0.05) association with nulliparity, nodal status, HER2 status, metastasis, HER2 enriched subtype and luminal A.
Conclusion
It is concluded that ESR1-rs2234693 is not associated with breast cancer, while rs2046210 is significantly associated with the risk of breast cancer in Khyber Pakhtunkhwa population. Further, to confirm the exact situation of ESR1 polymorphism, ESR1 existing and other SNPs need to be investigated in diverse data sets.
Similar content being viewed by others
Availability of data and materials
All the relevant data are included in the manuscript, related queries can be forward to the corresponding author.
References
Momenimovahed Z, Salehiniya H (2019) Epidemiological characteristics of and risk factors for breast cancer in the world. Breast cancer (Dove Med Press 11:151–164
Sharma GN, Dave R, Sanadya J, Sharma P, Sharma K (2010) Various types and management of breast cancer: an overview. J Adv Pharm Technol Res Medknow Publications 1:109
Nikolaou M, Pavlopoulou A, Georgakilas AG, Kyrodimos E (2018) The challenge of drug resistance in cancer treatment: a current overview. Clin Exp Metastasis Springer 35:309–318
Feng Y, Spezia M, Huang S, Yuan C, Zeng Z, Zhang L et al (2018) Breast cancer development and progression: Risk factors, cancer stem cells, signaling pathways, genomics, and molecular pathogenesis. Genes Dis [Internet]. Elsevier Ltd; 5:77–106. Available from: https://doi.org/10.1016/j.gendis.2018.05.001
Eroles P, Bosch A, Pérez-Fidalgo JA, Lluch A (2012) Molecular biology in breast cancer: intrinsic subtypes and signaling pathways. Cancer Treat Rev Elsevier 38:698–707
Rusidzé M, Adlanmérini M, Chantalat E, Raymond-Letron I, Cayre S, Arnal J-F et al (2021) Estrogen receptor-α signaling in post-natal mammary development and breast cancers. Cell Mol Life Sci Springer 78:5681–5705
Kudela E, Samec M, Koklesova L, Liskova A, Kubatka P, Kozubik E et al (2020) miRNA expression profiles in luminal A breast cancer—implications in biology, prognosis, and prediction of response to hormonal treatment. Int J Mol Sci MDPI 21:7691
García-Becerra R, Santos N, Díaz L, Camacho J (2012) Mechanisms of resistance to endocrine therapy in breast cancer: focus on signaling pathways, miRNAs and genetically based resistance. Int J Mol Sci Molecular Diversity Preservation International (MDPI) 14:108–145
Cheng SL, Lin H-JL, Huang TH-M (2008) Interrogating estrogen receptor α signaling in breast Cancer by chromatin immunoprecipitation microarrays. Genomics Endocrinol. Springer, pp 115–129
Shah AN, Metzger O, Bartlett CH, Liu Y, Huang X, Cristofanilli M (2020) Hormone receptor–positive/human epidermal growth receptor 2–negative metastatic breast cancer in young women: emerging data in the era of molecularly targeted agents, vol 25. Oxford University Press, Oncologist, pp e900–e908
Lima ZS, Ghadamzadeh M, Arashloo FT, Amjad G, Ebadi MR, Younesi L (2019) Recent advances of therapeutic targets based on the molecular signature in breast cancer: genetic mutations and implications for current treatment paradigms. J Hematol Oncol Springer 12:1–25
Shah M, Danish L, Khan NU, Zaman F, Ismail M, Hussain M et al (2020) Determination of mutations in iron regulating genes of beta thalassemia major patients of Khyber Pakhtunkhwa, Pakistan. Mol Genet Genomic Med 8:1–10
Adnan F, Khan NU, Iqbal A, Ali I, Petruzziello A, Sabatino R et al (2020) Interleukin-6 polymorphisms in HCC patients chronically infected with HCV. Infect Agent Cancer Infectious Agents and Cancer 15:1–7
Hayat F, Khan NU, Khan AU, Ahmad I, Alamri AM, Iftikhar B (2022) Risk association of RANKL and OPG gene polymorphism with breast cancer to bone metastasis in Pashtun population of Khyber Pakhtunkhwa, Pakistan. PLoS One [Internet]. ;17:e0276813. Available from: https://doi.org/10.1371/journal.pone.0276813
Jehanzeb M, Khan NU, Hussain M, Subrina J, Ayub S, Mustafa A (2022) Association of candidate genes (ALR2, RAGE, and VEGF) polymorphisms with diabetic retinopathy in type 2 diabetic patients of Khyber Pakhtunkhwa, Pakistan. Mol Biol Rep [Internet]. Springer Netherlands; Available from: https://doi.org/10.1007/s11033-022-08057-x
Jin C, Li Z, Zheng X, Shen K, Chao J, Dong Y et al (2020) Development and validation of T-ARMS-PCR to detect CYP2C19*17 allele. J Clin Lab Anal 34:1–5
Garbuz MM, Ovchinnikova AA, Kumeiko VV, Design (2022) Optimization and validation of the ARMS PCR protocol for the Rapid diagnosis of Wilson’s Disease using a panel of 14 common mutations for the European Population. Genes (Basel) 13:1940
Khera AV, Chaffin M, Aragam KG, Haas ME, Roselli C, Choi SH et al (2018) Genome-wide polygenic scores for common diseases identify individuals with risk equivalent to monogenic mutations. Nat Genet Nature Publishing Group 50:1219–1224
Mahdavi M, Nassiri M, Kooshyar MM, Vakili-Azghandi M, Avan A, Sandry R et al (2019) Hereditary breast cancer; genetic penetrance and current status with BRCA. J Cell Physiol Wiley Online Library 234:5741–5750
Siersbæk R, Kumar S, Carroll JS (2018) Signaling pathways and steroid receptors modulating estrogen receptor α function in breast cancer. Genes Dev Cold Spring Harbor Lab 32:1141–1154
Jeselsohn R, Buchwalter G, De Angelis C, Brown M, Schiff R (2015) ESR1 mutations-a mechanism for acquired endocrine resistance in breast cancer. Nat Rev Clin Oncol 12:573–583
Carrillo-Moreno DI, Figuera LE, González GMZ, Perez AMP, Mendoza AJM, Arreola MPG (2019) Association of rs2234693 and rs9340799 polymorphisms of ESR1 gene in breast cancer of mexican population. J BUON 24:1927–1933
Yang W, He X, He C, Peng L, Xing S, Li D et al (2021) Impact of ESR1 polymorphisms on risk of breast cancer in the Chinese han population. Clin Breast Cancer Elsevier 21:e235–e242
Iwasaki M, Hamada GS, Nishimoto IN, Netto MM, Motola J Jr, Laginha FM et al (2009) Isoflavone, polymorphisms in estrogen receptor genes and breast cancer risk in case-control studies in japanese, japanese brazilians and non‐japanese brazilians. Cancer Sci Wiley Online Library 100:927–933
Mirzaeyan P, Shokrzadeh M, Salehzadeh A, Ajamian F (2020) Association of estrogen receptor 1 (ESR1) gene (rs2234693) polymorphism, ESR1 promoter methylation status, and serum heavy metals concentration, with breast cancer: a study on iranian women population. Meta gene, vol 26. Elsevier, p 100802
Yang Z, Shen J, Cao Z, Wang B (2013) Association between a novel polymorphism (rs2046210) of the 6q25. 1 locus and breast cancer risk. Breast Cancer Res Treat Springer 139:267–275
Zheng W, Long J, Gao Y-T, Li C, Zheng Y, Xiang Y-B et al (2009) Genome-wide association study identifies a new breast cancer susceptibility locus at 6q25. 1. Nat Genet Nature Publishing Group 41:324–328
Thanh NTN, Lan NTT, Phat PT, Giang NDT, Hue NT (2018) Two polymorphisms, rs2046210 and rs3803662, are associated with breast cancer risk in a vietnamese case-control cohort.Genes Genet Syst. The Genetics Society of Japan; 17–53
Stacey SN, Sulem P, Zanon C, Gudjonsson SA, Thorleifsson G, Helgason A et al (2010) Ancestry-shift refinement mapping of the C6orf97-ESR1 breast cancer susceptibility locus. PLoS Genet 6:1–12
Acknowledgements
Not applicable.
Funding
There is no funding received for this work, the authors have contributed their self for experimental cost.
Author information
Authors and Affiliations
Contributions
SS collect the data, did the experiments and write the article. NUK and AQ designed and supervised the study. WS, RUS, AUK and IM facilitated the study, support the patient’s enrolment and review the article.
Corresponding author
Ethics declarations
Competing interests
The authors have no competing interest.
Ethical approval and consent to participate
Ethical approval was taken from the institute of Biotechnology and Genetic Engineering, and written consent was taken from all the participants after explaining the aim and objective of the study.
Consent for publication
All authors have read the article and agreed to publish the article.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Shahzad, S., Khan, N.U., Sombal, W. et al. Estrogen receptor alpha (ESR1) gene polymorphism (rs2234693 and rs2046210) with breast cancer risk in pashtun population of Khyber Pakhtunkhwa. Mol Biol Rep 50, 2445–2451 (2023). https://doi.org/10.1007/s11033-022-08198-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-022-08198-z