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Genetic polymorphism detection in brazilian perennial cottons (Gossypium spp.) using an ISSR marker system and its application for molecular interspecific differentiation

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Abstract

Background

The semi-domesticated Brazilian perennial cotton (Gossypium spp.) germplasm is considered a source of variability for creating modern upland cotton varieties. Here we used Inter-simple Sequence Repeat (ISSR) markers to detect intra and interspecific genetic polymorphism in Gossypium hirsutum L. r. marie-galante and Gossypium barbadense L. and to use molecular data to assessing genetic diversity and molecular discrimination of these species.

Methods and results

The sets contained 12 G. barbadense genotypes and 16 G. hirsutum genotypes from a Brazilian collection. The 11 ISSR primers were used for genotyping yielded 101 bands (polymorphism = 47.5%) and were classified as moderately informative (PIC = 0.304). The ISSR markers exposed a greater diversity in G. hirsutum (P = 24.72%; HE =0.071 and I = 0.111) as compared to G. barbadense (P = 17.98%, HE = 0.043 and I = 0.070). The AMOVA analysis showed that 89.47% of the genetic variation was partitioned within species which is supported by Nei’s genetic differentiation (Gst = 0.598) and gene flow (Nm = 0.338), suggesting that strong reproductive barriers between species. The UPGMA Cluster Analysis, Principal Coordinate Analysis and Bayesian Model-Based Structural Analysis divided the 28 genotypes into two main clades consistent with the taxonomical delimitation.

Conclusion

The ISSR marker system offers a new approach to determining molecular differences between two cotton species (G. hirsutum L. r. marie-galante and G. barbadense L.). This study can expand the molecular marker resources for the identification and improvement of our knowledge about the genetic diversity and relationships between perennial cotton genotypes.

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Acknowledgements

The authors express their gratitude to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brazil) for funding scholarships (Finance Code 001); Brazilian Agricultural Research Agency (Embrapa Cotton) for providing the germplasm and institutional support; and Kyvia Pontes for statistic support and her help in creating the artwork (the figures).

Funding

This study was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (Finance Code 001) and Brazilian Agricultural Research Agency (Brazil).

Author information

Authors and Affiliations

  1. Universidade Federal do Agreste de Pernambuco, Programa de Pós-Graduação em Produção Agrícola UFRPE, Av. Bom Pastor s/n – Boa Vista, 55292- 270, Garanhuns, Pernambuco, Brazil

    Fernando dos Santos Araújo & Alberício Pereira de Andrade

  2. Universidade Federal da Paraíba, Programa de Pós-Graduação em Agronomia, Areia, Paraíba, Brazil

    Riselane de Lucena Alcântara Bruno & Karialane da Silva Belarmino

  3. Brazilian Agricultural Research Corporation, EMBRAPA Cotton, Campina Grande, Paraíba, Brazil

    Nair Helena Castro Arriel, Everaldo Paulo de Medeiros & Liziane Maria de Lima

  4. Centro de Estudos Superiores de Maceió CESMAC, Maceió, Alagoas, Brazil

    Mayara Andrade de Souza

  5. Programa de Pós-Graduação em Ciências Florestais, Universidade Federal de Campina Grande, Patos, Paraíba, Brazil

    Richeliel Albert Rodrigues Silva

  6. Departamento de Engenharia Florestal, Universidade Federal do Paraná, Curitiba, Paraná, Brazil

    Francival Cardoso Felix

Authors
  1. Fernando dos Santos Araújo
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  2. Riselane de Lucena Alcântara Bruno
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  3. Nair Helena Castro Arriel
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  4. Everaldo Paulo de Medeiros
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  7. Alberício Pereira de Andrade
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  10. Karialane da Silva Belarmino
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Contributions

Fernando dos Santos Araújo, Riselane de Lucena Alcântara Bruno, Nair Helena Castro Arriel and Everaldo Paulo de Medeiros contributed to the study conception and design. Material preparation, data collection and analysis were performed by Liziane Maria de Lima and Mayara Andrade de Souza. The first draft of the manuscript was written by Fernando dos Santos Araújo, Riselane de Lucena Alcântara Bruno, Nair Helena Castro Arriel and Alberício Pereira de Andrade. Francival Cardoso Felix, Richeliel Albert Rodrigues Silva and Karialane da Silva Belarmino commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Fernando dos Santos Araújo.

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Araújo, F.d.S., Bruno, R.d.L.A., Arriel, N.H.C. et al. Genetic polymorphism detection in brazilian perennial cottons (Gossypium spp.) using an ISSR marker system and its application for molecular interspecific differentiation. Mol Biol Rep 50, 3001–3009 (2023). https://doi.org/10.1007/s11033-022-08165-8

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