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Induced expression of Ganoderma boninense Lanosterol 14α-Demethylase (ERG11) during interaction with oil palm

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Abstract

Background

The basidiomycete fungus, Ganoderma boninense is the main contributor to oil palm Basal Stem Rot (BSR) in Malaysia and Indonesia. Lanosterol 14α-Demethylase (ERG11) is a key enzyme involved in biosynthesis of ergosterol, which is an important component in the fungal cell membrane. The Azole group fungicides are effective against pathogenic fungi including G. boninense by inhibiting the ERG11 activity. However, the work on molecular characterization of G. boninense ERG11 is still unavailable today.

Methods and results

This study aimed to isolate and characterize the full-length cDNA encoding ERG11 from G. boninense. The G. boninense ERG11 gene expression during interaction with oil palm was also studied. A full-length 1860 bp cDNA encoding ERG11 was successfully isolated from G. boninense. The G. boninense ERG11 shared 91% similarity to ERG11 from other basidiomycete fungi. The protein structure homology modeling of GbERG11 was analyzed using the SWISS-MODEL workspace. Southern blot and genome data analyses showed that there is only a single copy of ERG11 gene in the G. boninense genome. Based on the in-vitro inoculation study, the ERG11 gene expression in G. boninense has shown almost 2-fold upregulation with the presence of oil palm.

Conclusion

This study provided molecular information and characterization study on the G. boninense ERG11 and this knowledge could be used to design effective control measures to tackle the BSR disease of oil palm.

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Acknowledgements

The authors would like to thank the management of MPOB for the approval and funding of this study. Our appreciation also goes to the Plant Pathology and Biosecurity (PPB) Unit, MPOB for providing G. boninense culture; Breeding and Tissue Culture Unit, MPOB for supplying the oil palm plantlets; Bioinformatics Unit, MPOB for the genome sequence data analysis; Transgenic Technology Group members especially Madam Siti Marlia Silong and Madam Nur Syazwana Shamsudin for their contributions in this study.

Funding

This study was supported by grant from the Malaysian Palm Oil Board (MPOB) under the project code of BD384-2009.

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Authors and Affiliations

  1. Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia

    Fook-Hwa Lim, Omar Abd Rasid, Abu Seman Idris & Ghulam Kadir Ahmad Parveez

  2. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Fook-Hwa Lim, Abdul Wahab Mohd As’wad, Ganesan Vadamalai & Mui-Yun Wong

  3. Institute of Plantation Studies, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Ganesan Vadamalai & Mui-Yun Wong

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  1. Fook-Hwa Lim
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by LFH, ROA and WMY. The first draft of the manuscript was written by LFH and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Fook-Hwa Lim or Mui-Yun Wong.

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Lim, FH., Rasid, O.A., Idris, A.S. et al. Induced expression of Ganoderma boninense Lanosterol 14α-Demethylase (ERG11) during interaction with oil palm. Mol Biol Rep 50, 2367–2379 (2023). https://doi.org/10.1007/s11033-022-08131-4

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